Isolation of <i>Pseudomonas fluorescens</i> Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia

Fekadu Alemu

doi:doi:10.11648/j.ajbio.20160402.11

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Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia

Fekadu Alemu

Published in American Journal of BioScience (Volume 4, Issue 2)

Received: 11 June 2015 Accepted: 19 June 2015 Published: 19 March 2016

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Abstract

The microbial inoculants that are used in agriculture include phytohormone synthesis which have role to increase crop growth and yield products. Some microbes have a potential to synthesize plant hormone through utilize the root exudates of plant in rhizospheric area. The objective of this study was to isolate some Pseudomonas fluorescens species from rhizospheric soil of faba bean and tested their indole acetic acid production. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. All isolates Pseudomonas fluorescens were tseted for indole acetic acid production have a potential to produce phytohormone. The test Pseudomonas fluorescens isolates culture were inoculated in the nutrient broth with L-tryptophan 500 mg/l at 28 ± 2°C for one week. The results indicated that all most of the isolates tested possess plant growth promoting traits (phytohormone). So it could be concluded that all isolate Pseudomonas fluorescens can be used as plant growth promoting bacteria for synthesis of plant hormone resulting to increase of the plant growth and yield.

Published in	American Journal of BioScience (Volume 4, Issue 2)
DOI	10.11648/j.ajbio.20160402.11
Page(s)	9-15
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Faba Bean, Indole Acetic Acid, Phytohormone, Plant Growth Promoting Rhizobacteria, Pseudomonas fluorescens

References

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APA Style

Fekadu Alemu. (2016). Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia. American Journal of BioScience, 4(2), 9-15. https://doi.org/10.11648/j.ajbio.20160402.11

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ACS Style

Fekadu Alemu. Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia. Am. J. BioScience 2016, 4(2), 9-15. doi: 10.11648/j.ajbio.20160402.11

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AMA Style

Fekadu Alemu. Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia. Am J BioScience. 2016;4(2):9-15. doi: 10.11648/j.ajbio.20160402.11

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@article{10.11648/j.ajbio.20160402.11,
  author = {Fekadu Alemu},
  title = {Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia},
  journal = {American Journal of BioScience},
  volume = {4},
  number = {2},
  pages = {9-15},
  doi = {10.11648/j.ajbio.20160402.11},
  url = {https://doi.org/10.11648/j.ajbio.20160402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20160402.11},
  abstract = {The microbial inoculants that are used in agriculture include phytohormone synthesis which have role to increase crop growth and yield products. Some microbes have a potential to synthesize plant hormone through utilize the root exudates of plant in rhizospheric area. The objective of this study was to isolate some Pseudomonas fluorescens species from rhizospheric soil of faba bean and tested their indole acetic acid production. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. All isolates Pseudomonas fluorescens were tseted for indole acetic acid production have a potential to produce phytohormone. The test Pseudomonas fluorescens isolates culture were inoculated in the nutrient broth with L-tryptophan 500 mg/l at 28 ± 2°C for one week. The results indicated that all most of the isolates tested possess plant growth promoting traits (phytohormone). So it could be concluded that all isolate Pseudomonas fluorescens can be used as plant growth promoting bacteria for synthesis of plant hormone resulting to increase of the plant growth and yield.},
 year = {2016}
}

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TY  - JOUR
T1  - Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia
AU  - Fekadu Alemu
Y1  - 2016/03/19
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbio.20160402.11
DO  - 10.11648/j.ajbio.20160402.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 9
EP  - 15
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20160402.11
AB  - The microbial inoculants that are used in agriculture include phytohormone synthesis which have role to increase crop growth and yield products. Some microbes have a potential to synthesize plant hormone through utilize the root exudates of plant in rhizospheric area. The objective of this study was to isolate some Pseudomonas fluorescens species from rhizospheric soil of faba bean and tested their indole acetic acid production. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. All isolates Pseudomonas fluorescens were tseted for indole acetic acid production have a potential to produce phytohormone. The test Pseudomonas fluorescens isolates culture were inoculated in the nutrient broth with L-tryptophan 500 mg/l at 28 ± 2°C for one week. The results indicated that all most of the isolates tested possess plant growth promoting traits (phytohormone). So it could be concluded that all isolate Pseudomonas fluorescens can be used as plant growth promoting bacteria for synthesis of plant hormone resulting to increase of the plant growth and yield.
VL  - 4
IS  - 2
ER  -

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Author Information

Fekadu Alemu

Department of Biology, Dilla University, College of Natural and Computational Sciences, Dilla, Ethiopia

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APA Style

Fekadu Alemu. (2016). Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia. American Journal of BioScience, 4(2), 9-15. https://doi.org/10.11648/j.ajbio.20160402.11

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ACS Style

Fekadu Alemu. Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia. Am. J. BioScience 2016, 4(2), 9-15. doi: 10.11648/j.ajbio.20160402.11

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AMA Style

Fekadu Alemu. Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia. Am J BioScience. 2016;4(2):9-15. doi: 10.11648/j.ajbio.20160402.11

Copy | Download

@article{10.11648/j.ajbio.20160402.11,
  author = {Fekadu Alemu},
  title = {Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia},
  journal = {American Journal of BioScience},
  volume = {4},
  number = {2},
  pages = {9-15},
  doi = {10.11648/j.ajbio.20160402.11},
  url = {https://doi.org/10.11648/j.ajbio.20160402.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20160402.11},
  abstract = {The microbial inoculants that are used in agriculture include phytohormone synthesis which have role to increase crop growth and yield products. Some microbes have a potential to synthesize plant hormone through utilize the root exudates of plant in rhizospheric area. The objective of this study was to isolate some Pseudomonas fluorescens species from rhizospheric soil of faba bean and tested their indole acetic acid production. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. All isolates Pseudomonas fluorescens were tseted for indole acetic acid production have a potential to produce phytohormone. The test Pseudomonas fluorescens isolates culture were inoculated in the nutrient broth with L-tryptophan 500 mg/l at 28 ± 2°C for one week. The results indicated that all most of the isolates tested possess plant growth promoting traits (phytohormone). So it could be concluded that all isolate Pseudomonas fluorescens can be used as plant growth promoting bacteria for synthesis of plant hormone resulting to increase of the plant growth and yield.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Isolation of Pseudomonas fluorescens Species from faba Bean Rhizospheric Soil and Assessment of Indole Acetic Acid Production: In Vitro Study, Ethiopia
AU  - Fekadu Alemu
Y1  - 2016/03/19
PY  - 2016
N1  - https://doi.org/10.11648/j.ajbio.20160402.11
DO  - 10.11648/j.ajbio.20160402.11
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 9
EP  - 15
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20160402.11
AB  - The microbial inoculants that are used in agriculture include phytohormone synthesis which have role to increase crop growth and yield products. Some microbes have a potential to synthesize plant hormone through utilize the root exudates of plant in rhizospheric area. The objective of this study was to isolate some Pseudomonas fluorescens species from rhizospheric soil of faba bean and tested their indole acetic acid production. Isolation of Pseudomonas fluorescens isolates were carried out on King’s B medium. All isolates Pseudomonas fluorescens were tseted for indole acetic acid production have a potential to produce phytohormone. The test Pseudomonas fluorescens isolates culture were inoculated in the nutrient broth with L-tryptophan 500 mg/l at 28 ± 2°C for one week. The results indicated that all most of the isolates tested possess plant growth promoting traits (phytohormone). So it could be concluded that all isolate Pseudomonas fluorescens can be used as plant growth promoting bacteria for synthesis of plant hormone resulting to increase of the plant growth and yield.
VL  - 4
IS  - 2
ER  -

Copy | Download