Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat

Monteomo Gnate François; Koffi Dago Pierre; Atto Virginie; Deh Zhou Patricia; Yapi Ahoua

doi:doi:10.11648/j.ajbio.20160402.12

| Peer-Reviewed

Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat

Monteomo Gnate François, Koffi Dago Pierre, Atto Virginie, Deh Zhou Patricia, Yapi Ahoua

Published in American Journal of BioScience (Volume 4, Issue 2)

Received: 1 February 2016 Accepted: 18 March 2016 Published: 3 May 2016

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Our aim was to expose a method of study an antihyperglycemic traditional cure with oral glucose tolerance test to wistar rat. This methodology began by obtaining plants species component the cure, their extraction and identification of assets. The traditional cure is composited of leaves of both Heliotropium indicum (Borraginaceae), Ocimum gratissimum (Lamiaceae) and barks of both Sclerocarya birrea, (Anacardiaceae), Khaya senegalensis (Meliaceae). After, experimental animal is chosen and the blood samples are made. The pharmacological criteria took into account the oral antidiabetic therapeutic as the glibenclamide. Then the glucose overload is absorbed by the animals after administration of the test cure in single or multiple outlets. The anhydrous glucose used to induce experimental diabetes is administered at a dose of 4 g/kg/vo. Finally, we carried out the blood glucose. The methodology of study of an antihyperglycemic traditional cure took account the protection of laboratory animals after experimentation.

Published in	American Journal of BioScience (Volume 4, Issue 2)
DOI	10.11648/j.ajbio.20160402.12
Page(s)	16-19
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Methodology, Traditional Cure, Oral Glucose, Wistar Rat

References

[1]	Aké AL. Abrégés de médecine et pharmacopée ivoiriennes: quelques plantes employées, dans les soins de santé primaire. Editions NEI-CEDA. Abidjan, 2012, 86-94.
[2]	Anitha M, Sakthidevi G, Muthukumarasamy S, Mohan VR. Effect of cynoglossum zeylanicum (Vehl ex Hornem) Thinb. ex Lehm on oral glucose tolerance in rats. J of Applied pharmaceutical Science, 2012; 2, 11: 75-78.
[3]	Bauck. Basic anatomy, physiology, husbandry and clinical techniques. Part I. In Hillyer, Quesenberry. Ferrets, rabbits and roddents. Clin med and surg, saunders, 2004; 26, 291-297.
[4]	Debuigue G. Larousse des plantes qui guérissent. Librairie Larousse, 2010; p. 5-6.
[5]	Keita A, Mariko E, Haidara TK. Etude de l'activité hypoglycémiante des feuilles de Sclerocarya birrea (A. Rich) Hochst. (Anacardiaceae) Pharm. Méd. Trad. Afr. 1998, 10: 16-25.
[6]	Kerharo J, Adam JC. La pharmacopée sénégalaise traditionnelle: plantes médicinales et tropicales. Paris, Ed Vigot et frères, 1974; 433-35.
[7]	Konkon NG, Adjoungoua AL, Manda P. Toxicological and phytochemical screening study of Mitragyna inermis (Rubiaceae). Antidiabetic plant. J of medecinal plants research, 2008; 2, 10: 279-284.
[8]	Lokrou A. Guide de prise en charge des diabétiques. Collection santé, Editions Universitaires de Côte d’Ivoire, 2008; 212p.
[9]	Mohamed A, Tanko Y, Okasha MA. Effects of aqueous leaves extract of Ocimum gratissimum on blood glucose levels of streptozocin-induced diabetic Wistar rats. Afr J Biotechnolog, 2007; 6, 18: 2087-90.
[10]	Monteomo GF, Tehoua L, Ouattara H et al. Comparative antihyperglycemic activity of Sclerocarya birrea, Khaya senegalensis, Heliotropium indicum and Ocimum gratissimum to rats Wistar. Am J Biosciences, 2014; 2, 2: 60-63.
[11]	Monteomo GF. Valorisation de la pharmacopée africaine: étude de l’activité antihyperglycémique d’une préparation traditionnelle améliorée composée de sclerocarya birrea (Anacardiacée), Khaya senegalensis (Meliacée), Heliotropium indicum (Borraginacée) et Ocimum gratissimum (Lamiacée). Doctorat des Universités, Option, Biologie Humaine, Université FHB-Abidjan, UFR-SM, 2015; N° 26, 172p.
[12]	Nemlin J, Brunel JF. Travaux pratiques de matière médicale, 3^ème année, Paris, Masson, 1995-1996: 39-43.
[13]	Ojewole JAO. Laboratory evaluation of the hypoglycemic effect of anarcadium occidentale Linn. (Anarcadiaceae) stem-bark extracts in rats. Methods and findings in experimental and clinical, 2003; 25, 3: 199-204.
[14]	Oyedemi S, Bradley G, Afolayan A. Antidiabetic activities of aqueous stem bark extract of Strychnos henningsii Gild in streptozotocin-nicotinamide types 2 diabetic rats. J of Pharmaceutical Research, 2012; 11, 1: 221-228.
[15]	Vilmer JB. Ethique animale. Que sais-je. Presses universitaires de France, Paris, 2015; 79-90p.
[16]	Zhao VW, James MU, Schraw TD. Panic-Attac: A Mouse model for Inducible and Reversible β-Cell Ablation. Diab. 2008; 57, 8: 2137-2148.

Cite This Article

Plain Text BibTeX RIS

APA Style

Monteomo Gnate François, Koffi Dago Pierre, Atto Virginie, Deh Zhou Patricia, Yapi Ahoua. (2016). Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. American Journal of BioScience, 4(2), 16-19. https://doi.org/10.11648/j.ajbio.20160402.12

Copy | Download

ACS Style

Monteomo Gnate François; Koffi Dago Pierre; Atto Virginie; Deh Zhou Patricia; Yapi Ahoua. Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. Am. J. BioScience 2016, 4(2), 16-19. doi: 10.11648/j.ajbio.20160402.12

Copy | Download

AMA Style

Monteomo Gnate François, Koffi Dago Pierre, Atto Virginie, Deh Zhou Patricia, Yapi Ahoua. Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. Am J BioScience. 2016;4(2):16-19. doi: 10.11648/j.ajbio.20160402.12

Copy | Download

@article{10.11648/j.ajbio.20160402.12,
  author = {Monteomo Gnate François and Koffi Dago Pierre and Atto Virginie and Deh Zhou Patricia and Yapi Ahoua},
  title = {Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat},
  journal = {American Journal of BioScience},
  volume = {4},
  number = {2},
  pages = {16-19},
  doi = {10.11648/j.ajbio.20160402.12},
  url = {https://doi.org/10.11648/j.ajbio.20160402.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20160402.12},
  abstract = {Our aim was to expose a method of study an antihyperglycemic traditional cure with oral glucose tolerance test to wistar rat. This methodology began by obtaining plants species component the cure, their extraction and identification of assets. The traditional cure is composited of leaves of both Heliotropium indicum (Borraginaceae), Ocimum gratissimum (Lamiaceae) and barks of both Sclerocarya birrea, (Anacardiaceae), Khaya senegalensis (Meliaceae). After, experimental animal is chosen and the blood samples are made. The pharmacological criteria took into account the oral antidiabetic therapeutic as the glibenclamide. Then the glucose overload is absorbed by the animals after administration of the test cure in single or multiple outlets. The anhydrous glucose used to induce experimental diabetes is administered at a dose of 4 g/kg/vo. Finally, we carried out the blood glucose. The methodology of study of an antihyperglycemic traditional cure took account the protection of laboratory animals after experimentation.},
 year = {2016}
}

Copy | Download

TY - JOUR
T1 - Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat
AU - Monteomo Gnate François
AU - Koffi Dago Pierre
AU - Atto Virginie
AU - Deh Zhou Patricia
AU - Yapi Ahoua
Y1 - 2016/05/03
PY - 2016
N1 - https://doi.org/10.11648/j.ajbio.20160402.12
DO - 10.11648/j.ajbio.20160402.12
T2 - American Journal of BioScience
JF - American Journal of BioScience
JO - American Journal of BioScience
SP - 16
EP - 19
PB - Science Publishing Group
SN - 2330-0167
UR - https://doi.org/10.11648/j.ajbio.20160402.12
AB - Our aim was to expose a method of study an antihyperglycemic traditional cure with oral glucose tolerance test to wistar rat. This methodology began by obtaining plants species component the cure, their extraction and identification of assets. The traditional cure is composited of leaves of both Heliotropium indicum (Borraginaceae), Ocimum gratissimum (Lamiaceae) and barks of both Sclerocarya birrea, (Anacardiaceae), Khaya senegalensis (Meliaceae). After, experimental animal is chosen and the blood samples are made. The pharmacological criteria took into account the oral antidiabetic therapeutic as the glibenclamide. Then the glucose overload is absorbed by the animals after administration of the test cure in single or multiple outlets. The anhydrous glucose used to induce experimental diabetes is administered at a dose of 4 g/kg/vo. Finally, we carried out the blood glucose. The methodology of study of an antihyperglycemic traditional cure took account the protection of laboratory animals after experimentation.
VL - 4
IS - 2
ER -

Copy | Download

Author Information

Monteomo Gnate François
Koffi Dago Pierre

Endocrinology and Diabetology Service, CHU-Yopougon-Abidjan, Abidjan, C?te d’Ivoire
Atto Virginie

Laboratory of Animal Physiology, UFR-Biosciences-University Felix Houphouēt-Boigny, Abidjan, C?te d’Ivoire
Deh Zhou Patricia

Laboratory of Embryol-Cytol-Cytogen UFR-Med. Sciences-Univ F Houphouēt-Boigny, Abidjan, C?te d’Ivoire
Yapi Ahoua

Laboratory of Animal Biology, UFR-Biosciences-University Felix Houphouēt-Boigny, Abidjan, C?te d’Ivoire

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Monteomo Gnate François, Koffi Dago Pierre, Atto Virginie, Deh Zhou Patricia, Yapi Ahoua. (2016). Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. American Journal of BioScience, 4(2), 16-19. https://doi.org/10.11648/j.ajbio.20160402.12

Copy | Download

ACS Style

Monteomo Gnate François; Koffi Dago Pierre; Atto Virginie; Deh Zhou Patricia; Yapi Ahoua. Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. Am. J. BioScience 2016, 4(2), 16-19. doi: 10.11648/j.ajbio.20160402.12

Copy | Download

AMA Style

Monteomo Gnate François, Koffi Dago Pierre, Atto Virginie, Deh Zhou Patricia, Yapi Ahoua. Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat. Am J BioScience. 2016;4(2):16-19. doi: 10.11648/j.ajbio.20160402.12

Copy | Download

@article{10.11648/j.ajbio.20160402.12,
  author = {Monteomo Gnate François and Koffi Dago Pierre and Atto Virginie and Deh Zhou Patricia and Yapi Ahoua},
  title = {Methodology of Study of an Antihyperglycemic Traditional Cure with Oral Glucose Tolerance Test to Wistar Rat},
  journal = {American Journal of BioScience},
  volume = {4},
  number = {2},
  pages = {16-19},
  doi = {10.11648/j.ajbio.20160402.12},
  url = {https://doi.org/10.11648/j.ajbio.20160402.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20160402.12},
  abstract = {Our aim was to expose a method of study an antihyperglycemic traditional cure with oral glucose tolerance test to wistar rat. This methodology began by obtaining plants species component the cure, their extraction and identification of assets. The traditional cure is composited of leaves of both Heliotropium indicum (Borraginaceae), Ocimum gratissimum (Lamiaceae) and barks of both Sclerocarya birrea, (Anacardiaceae), Khaya senegalensis (Meliaceae). After, experimental animal is chosen and the blood samples are made. The pharmacological criteria took into account the oral antidiabetic therapeutic as the glibenclamide. Then the glucose overload is absorbed by the animals after administration of the test cure in single or multiple outlets. The anhydrous glucose used to induce experimental diabetes is administered at a dose of 4 g/kg/vo. Finally, we carried out the blood glucose. The methodology of study of an antihyperglycemic traditional cure took account the protection of laboratory animals after experimentation.},
 year = {2016}
}

Copy | Download