Effect of High Concentrations of CO<sub>2</sub> on the Cell Density, Lipid Content and Fatty Acid Components of <i>Nannochloropsis oceanica</i>

Wang Yuejie; Meng Fanping; Cui Hongwu; Duan Weiyan; Yi Xiaoyan

doi:doi:10.11648/j.sd.20160402.21

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Effect of High Concentrations of CO₂ on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica

Wang Yuejie, Meng Fanping, Cui Hongwu, Duan Weiyan, Yi Xiaoyan

Published in Science Discovery (Volume 4, Issue 2)

Received: 17 May 2016 Published: 18 May 2016

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Abstract

In order to evaluate the capability of Nannochloropsis oceanica to product biodiesel, the cell density, lipid content and fatty acid components of N. oceanica under different CO₂ concentrations were analized. The results indicated that the cell density and lipid content of N. oceanica were increased under 5% CO₂, but the growth of N. oceanica was inhibited under 10% and 15% CO₂. Over 88% of the N. oceanica lipids produced consisted of C16 ~ C18 fatty acids and the concentration of unsaturated fatty acids (>69%) is high and suitable for biofuel production. The highest content of eicosapentaenoic acid (EPA) was also obtained under 5% CO₂. The present results suggested that N. oceanica was suitable biodiesel feedstock and the maximum economic effectiveness will be obtained using flue gas containing 5% CO₂ in the cultivation of N. oceanica.

Published in	Science Discovery (Volume 4, Issue 2)
DOI	10.11648/j.sd.20160402.21
Page(s)	122-128
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Nannochloropsis oceanica, CO₂ Concentrations, Fatty Acid Components, Biodiesel, EPA

References

[1]	Milano J, Ong H C, Masjuki H H, et al. Microalgae biofuels as an alternative to fossil fuel for power generation [J]. Renewable and Sustainable Energy Reviews, 2016, 58: 180-197.
[2]	Talebi A F, Mohtashami S K, Tabatabaei M, et al. Fatty acids profiling: a selective criterion for screening microalgae strains for biodiesel production [J]. Algal Research, 2013, 2(3): 258-267.
[3]	Demirbas A. Progress and recent trends in biodiesel fuels [J]. Energy Conversion and Management, 2009, 50(1): 14-34.
[4]	吴立柱,窦世娟,从均广,等.我国微藻生物柴油的研究背景与发展战略[J].生物技术进展,2015,02:85-88。
[5]	周继如,朱世安.微藻生物柴油研究现状[J].能源环境保护,2015,01:50-53。
[6]	Batan L, Quinn J, Willson B, et al. Net energy and greenhouse gas emission evaluation of biodiesel derived from microalgae [J]. Environmental Science & Technology, 2010, 44(20): 7975-7980.
[7]	Liang Y, Sarkany N, Cui Y, et al. Biomass and lipid productivities of Chlorella vulgaris under autotrophic, heterotrophic and mixotrophic growth conditions [J]. Biotechnology Letters, 2009, 31(7): 1043-1049.
[8]	Zhao B, Su Y. Process effect of microalgal-carbon dioxide fixation and biomass production: a review [J]. Renewable and Sustainable Energy Reviews, 2014, 31: 121-132.
[9]	Gouveia L. Microalgae as a Feedstock for Biofuels [M]. Springer Berlin Heidelberg, 2011.
[10]	Lam M K, Lee K T, Mohamed A R. Current status and challenges on microalgae-based carbon capture [J]. International Journal of Greenhouse Gas Control, 2012, 10: 456-469.
[11]	Cheah W Y, Show P L, Chang J S, et al. Biosequestration of atmospheric CO₂ and flue gas-containing CO₂ by microalgae[J]. Bioresource Technology, 2015, 184: 190-201.
[12]	Yoshihara K I, Nagase H, Eguchi K, et al. Biological elimination of nitric oxide and carbon dioxide from flue gas by marine microalga NOA-113 cultivated in a long tubular photobioreactor [J]. Journal of Fermentation and Bioengineering, 1996, 82(4): 351-354.
[13]	Mudimu O, Rybalka N, Bauersachs T, et al. Influence of Different CO₂ Concentrations on Microalgae Growth, α-Tocopherol Content and Fatty Acid Composition [J]. Geomicrobiology Journal, 2015, 32(3-4): 291-303.
[14]	Ma Y, Wang Z, Zhu M, et al. Increased lipid productivity and TAG content in Nannochloropsis by heavy-ion irradiation mutagenesis [J]. Bioresource Technology, 2013, 136: 360-367.
[15]	Ma Y, Wang Z, Yu C, et al. Evaluation of the potential of 9 Nannochloropsis strains for biodiesel production [J]. Bioresource Technology, 2014, 167: 503-509.
[16]	李秀波,徐旭东,孔任秋.五种微绿球藻产油和产多不饱和脂肪酸的研究[J].水生生物学报,2010,05:893-897.
[17]	Chen C Y, Chen Y C, Huang H C, et al. Engineering strategies for enhancing the production of eicosapentaenoic acid (EPA) from an isolated microalga Nannochloropsis oceanica CY2 [J]. Bioresource Technology, 2013, 147: 160-167.
[18]	Malakootian M, Hatami B, Dowlatshahi S, et al. Optimization of culture media for lipid production by Nannochloropsis oculata for Biodiesel production [J]. Environmental Health Engineering and Management Journal, 2015, 2(3): 141-147.
[19]	Vieler A, Wu G, Tsai C H, et al. Genome, functional gene annotation, and nuclear transformation of the heterokont oleaginous alga Nannochloropsis oceanica CCMP1779 [J]. PLoS Genetics, 2012, 8(11): e1003064.
[20]	Guillard R R, Ryther J H. Studies of marine planktonic diatoms: I. cyclotella nana hustedt and detonula confervacea (cleve) [J]. Canadian Journal of Microbiology, 1962, 8(2): 229-239.
[21]	Bligh E G, Dyer W J. A rapid method of total lipid extraction and purification [J]. Canadian Journal of Biochemistry and Physiology, 1959, 37(8): 911-917.
[22]	Chen W, Sommerfeld M, Hu Q. Microwave-assisted Nile red method for in vivo quantification of neutral lipids in microalgae [J]. Bioresource Technology, 2011, 102(1): 135-141.
[23]	李高阳,丁霄霖.亚麻籽油中脂肪酸成分的GC-MS分析[J].食品与机械,2005,21(5):30-32。
[24]	Miyachi S, Iwasaki I, Shiraiwa Y. Historical perspective on microalgal and cyanobacterial acclimation to low-and extremely high-CO₂ conditions [J]. Photosynthesis Research, 2003, 77: 139-153.
[25]	李凤娟,万秀,方仙桃,等.不同氮源及CO₂浓度下湖泊微拟球藻的生长及产油特性分析[J] 水生生物学报,2015,39(2):436-440。
[26]	Chiu S Y, Kao C Y, Tsai M T, et al. Lipid accumulation and CO₂ utilization of Nannochloropsis oculata in response to CO₂ aeration [J]. Bioresource Technology, 2009, 100(2): 833-838.
[27]	Razzak S A, Ilyas M, Ali S A M, et al. Effects of CO₂ Concentration and pH on Mixotrophic Growth of Nannochloropsis oculata [J]. Applied Biochemistry and Biotechnology, 2015, 176(5): 1290-1302.
[28]	李林,郑立,郑明刚,等.富碳培养对海洋富油微藻油脂积累特性的影响[J].水生生物学报,2013,06:1013-1019。
[29]	Wijffels R H, Barbosa M J. An outlook on microalgal biofuels [J]. Science, 2010: 329: 796-799.
[30]	王曰杰,孟范平,李永富,等.内置LED光源平板型光生物反应器用于微藻培养——普通小球藻在反应器中的固碳产油性能探究[J]. 中国环境科学,2015,05:1526-1534。
[31]	Knothe G. Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters [J]. Fuel Processing Technology, 2005, 86(10): 1059-1070.
[32]	Knothe G. Improving biodiesel fuel properties by modifying fatty ester composition [J]. Energy & Environmental Science, 2009, 2(7): 759-766.
[33]	Lam M K, Lee K T, Mohamed A R. Current status and challenges on microalgae-based carbon capture [J]. International Journal of Greenhouse Gas Control, 2012, 10: 456-469.
[34]	Doan T T Y, Sivaloganathan B, Obbard J P. Screening of marine microalgae for biodiesel feedstock [J]. Biomass and Bioenergy, 2011, 35(7): 2534-2544.
[35]	Schenk P M, Thomas-Hall S R, Stephens E, et al. Second generation biofuels: high-efficiency microalgae for biodiesel production [J]. Bioenergy Research, 2008, 1(1): 20-43.
[36]	Tang D, Han W, Li P, et al. CO₂ biofixation and fatty acid composition of Scenedesmus obliquus and Chlorella pyrenoidosa in response to different CO2 levels [J]. Bioresource Technology, 2011, 102(3): 3071-3076.
[37]	Tan C K, Johns M R. Screening of diatoms for heterotrophic eicosapentaenoic acid production [J]. Journal of Applied Phycology, 1996, 8(1): 59-64.
[38]	徐芳,胡晗华,丛威,等.通气量和CO₂对Nannochloropsis sp.在光生物反应器中的生长和EPA合成的影响[J].过程工程学报,2004,05:457-461。
[39]	Kromkamp J C, Beardall J, Sukenik A, et al. Short-term variations in photosynthetic parameters of Nannochloropsis cultures grown in two types of outdoor mass cultivation systems [J]. Aquatic Microbial Ecology, 2009, 56(2-3): 309-322.
[40]	Hu H, Gao K. Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources [J]. Biotechnology Letters, 2003, 25(5): 421-425.
[41]	Hoshida H, Ohira T, Minematsu A, et al. Accumulation of eicosapentaenoic acid in Nannochloropsis sp. in response to elevated CO₂ concentrations [J]. Journal of Applied Phycology, 2005, 17(1): 29-34.

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APA Style

Wang Yuejie, Meng Fanping, Cui Hongwu, Duan Weiyan, Yi Xiaoyan. (2016). Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica. Science Discovery, 4(2), 122-128. https://doi.org/10.11648/j.sd.20160402.21

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ACS Style

Wang Yuejie; Meng Fanping; Cui Hongwu; Duan Weiyan; Yi Xiaoyan. Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica. Sci. Discov. 2016, 4(2), 122-128. doi: 10.11648/j.sd.20160402.21

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AMA Style

Wang Yuejie, Meng Fanping, Cui Hongwu, Duan Weiyan, Yi Xiaoyan. Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica. Sci Discov. 2016;4(2):122-128. doi: 10.11648/j.sd.20160402.21

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@article{10.11648/j.sd.20160402.21,
  author = {Wang Yuejie and Meng Fanping and Cui Hongwu and Duan Weiyan and Yi Xiaoyan},
  title = {Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica},
  journal = {Science Discovery},
  volume = {4},
  number = {2},
  pages = {122-128},
  doi = {10.11648/j.sd.20160402.21},
  url = {https://doi.org/10.11648/j.sd.20160402.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160402.21},
  abstract = {In order to evaluate the capability of Nannochloropsis oceanica to product biodiesel, the cell density, lipid content and fatty acid components of N. oceanica under different CO2 concentrations were analized. The results indicated that the cell density and lipid content of N. oceanica were increased under 5% CO2, but the growth of N. oceanica was inhibited under 10% and 15% CO2. Over 88% of the N. oceanica lipids produced consisted of C16 ~ C18 fatty acids and the concentration of unsaturated fatty acids (>69%) is high and suitable for biofuel production. The highest content of eicosapentaenoic acid (EPA) was also obtained under 5% CO2. The present results suggested that N. oceanica was suitable biodiesel feedstock and the maximum economic effectiveness will be obtained using flue gas containing 5% CO2 in the cultivation of N. oceanica.},
 year = {2016}
}

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TY  - JOUR
T1  - Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica
AU  - Wang Yuejie
AU  - Meng Fanping
AU  - Cui Hongwu
AU  - Duan Weiyan
AU  - Yi Xiaoyan
Y1  - 2016/05/18
PY  - 2016
N1  - https://doi.org/10.11648/j.sd.20160402.21
DO  - 10.11648/j.sd.20160402.21
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 122
EP  - 128
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20160402.21
AB  - In order to evaluate the capability of Nannochloropsis oceanica to product biodiesel, the cell density, lipid content and fatty acid components of N. oceanica under different CO2 concentrations were analized. The results indicated that the cell density and lipid content of N. oceanica were increased under 5% CO2, but the growth of N. oceanica was inhibited under 10% and 15% CO2. Over 88% of the N. oceanica lipids produced consisted of C16 ~ C18 fatty acids and the concentration of unsaturated fatty acids (>69%) is high and suitable for biofuel production. The highest content of eicosapentaenoic acid (EPA) was also obtained under 5% CO2. The present results suggested that N. oceanica was suitable biodiesel feedstock and the maximum economic effectiveness will be obtained using flue gas containing 5% CO2 in the cultivation of N. oceanica.
VL  - 4
IS  - 2
ER  -

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Author Information

Wang Yuejie

College of Environmental Science and Engineering, Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, China
Meng Fanping

College of Environmental Science and Engineering, Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, China
Cui Hongwu

College of Environmental Science and Engineering, Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, China
Duan Weiyan

College of Environmental Science and Engineering, Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, China
Yi Xiaoyan

College of Environmental Science and Engineering, Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao, China

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APA Style

Wang Yuejie, Meng Fanping, Cui Hongwu, Duan Weiyan, Yi Xiaoyan. (2016). Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica. Science Discovery, 4(2), 122-128. https://doi.org/10.11648/j.sd.20160402.21

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ACS Style

Wang Yuejie; Meng Fanping; Cui Hongwu; Duan Weiyan; Yi Xiaoyan. Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica. Sci. Discov. 2016, 4(2), 122-128. doi: 10.11648/j.sd.20160402.21

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AMA Style

Wang Yuejie, Meng Fanping, Cui Hongwu, Duan Weiyan, Yi Xiaoyan. Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica. Sci Discov. 2016;4(2):122-128. doi: 10.11648/j.sd.20160402.21

Copy | Download

@article{10.11648/j.sd.20160402.21,
  author = {Wang Yuejie and Meng Fanping and Cui Hongwu and Duan Weiyan and Yi Xiaoyan},
  title = {Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica},
  journal = {Science Discovery},
  volume = {4},
  number = {2},
  pages = {122-128},
  doi = {10.11648/j.sd.20160402.21},
  url = {https://doi.org/10.11648/j.sd.20160402.21},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sd.20160402.21},
  abstract = {In order to evaluate the capability of Nannochloropsis oceanica to product biodiesel, the cell density, lipid content and fatty acid components of N. oceanica under different CO2 concentrations were analized. The results indicated that the cell density and lipid content of N. oceanica were increased under 5% CO2, but the growth of N. oceanica was inhibited under 10% and 15% CO2. Over 88% of the N. oceanica lipids produced consisted of C16 ~ C18 fatty acids and the concentration of unsaturated fatty acids (>69%) is high and suitable for biofuel production. The highest content of eicosapentaenoic acid (EPA) was also obtained under 5% CO2. The present results suggested that N. oceanica was suitable biodiesel feedstock and the maximum economic effectiveness will be obtained using flue gas containing 5% CO2 in the cultivation of N. oceanica.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Effect of High Concentrations of CO2 on the Cell Density, Lipid Content and Fatty Acid Components of Nannochloropsis oceanica
AU  - Wang Yuejie
AU  - Meng Fanping
AU  - Cui Hongwu
AU  - Duan Weiyan
AU  - Yi Xiaoyan
Y1  - 2016/05/18
PY  - 2016
N1  - https://doi.org/10.11648/j.sd.20160402.21
DO  - 10.11648/j.sd.20160402.21
T2  - Science Discovery
JF  - Science Discovery
JO  - Science Discovery
SP  - 122
EP  - 128
PB  - Science Publishing Group
SN  - 2331-0650
UR  - https://doi.org/10.11648/j.sd.20160402.21
AB  - In order to evaluate the capability of Nannochloropsis oceanica to product biodiesel, the cell density, lipid content and fatty acid components of N. oceanica under different CO2 concentrations were analized. The results indicated that the cell density and lipid content of N. oceanica were increased under 5% CO2, but the growth of N. oceanica was inhibited under 10% and 15% CO2. Over 88% of the N. oceanica lipids produced consisted of C16 ~ C18 fatty acids and the concentration of unsaturated fatty acids (>69%) is high and suitable for biofuel production. The highest content of eicosapentaenoic acid (EPA) was also obtained under 5% CO2. The present results suggested that N. oceanica was suitable biodiesel feedstock and the maximum economic effectiveness will be obtained using flue gas containing 5% CO2 in the cultivation of N. oceanica.
VL  - 4
IS  - 2
ER  -

Copy | Download