Toxicity Study of <i>Pittosporum ochrosiaefolium</i> Bojer (Pittosporaceae) a Medicinal Plant of Madagascar

Maholy Pricille Ratsimiebo; David Ramanitrahasimbola; Clara Fredeline Rajemiarimoelisoa; Zoarilala Rinah Razafindrakoto; Hanitra Ranjana Randrianarivo; Danielle Aurore Doll Rakoto; Victor Louis Jeannoda

doi:doi:10.11648/j.jps.20150306.19

Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar

Maholy Pricille Ratsimiebo, David Ramanitrahasimbola, Clara Fredeline Rajemiarimoelisoa, Zoarilala Rinah Razafindrakoto, Hanitra Ranjana Randrianarivo, Danielle Aurore Doll Rakoto, Victor Louis Jeannoda

Published in Journal of Plant Sciences (Volume 3, Issue 6)

Received: 10 November 2015 Accepted: 20 November 2015 Published: 10 December 2015

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Abstract

The present work aimed to assess the leaf toxicity of Pittosporum ochrosiaefolium Bojer, a well-known medicinal plant endemic to Madagascar. Leaf methanolic extract (LME), obtained after successive extractions by hexan and methanol, was tested in vivo on warm and cold-blooded animals and in vitro on isolated atria of guinea-pig. LME was toxic to mice with a LD₅₀ of about 46.69 mg/kg of body weight by intraperitoneal route. It induced mainly nervous disorders (body fasciculation, clonic convulsions), respiratory troubles (reduction of respiration frequency and cyanosis) and diarrheas. By intraperitoneal route, LME (46.69 mg/kg) caused histopathological lesions in lungs, liver, kidneys, small and large intestines but had no effects on brain, heart and stomach. Vascular congestion, inflammatory infiltrates, edema and necrosis were frequently observed. LME had a positive inotropic effect but no significant chronotropic one on isolated atria. It did not alter renal and hepatic functions at 21.24 mg/kg. It was highly toxic to the frog Ptychadena mascareniensis (LC₅₀ of 13.51 µg/mL) and the fish Cyprinus carpio (LC₅₀ of 8.2 µg/mL). It was also toxic to mosquito larvae Culex quinquefasciatus and Aedes albopictus with LC₅₀ of 720 ppm and 910 ppm respectively. Different chemical compound groups were found in LME but only saponins proved to be toxic. Under certain conditions, P. ochrosiaefolium might be exploited as source of pesticides or therapeutic molecules.

Published in	Journal of Plant Sciences (Volume 3, Issue 6)
DOI	10.11648/j.jps.20150306.19
Page(s)	349-357
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Pittosporum ochrosiaefolium, Leaf Methanolic Extract, Saponins, Toxicity, Histopathological Lesions, Pharmacological Activity

References

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Maholy Pricille Ratsimiebo, David Ramanitrahasimbola, Clara Fredeline Rajemiarimoelisoa, Zoarilala Rinah Razafindrakoto, Hanitra Ranjana Randrianarivo, et al. (2015). Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar. Journal of Plant Sciences, 3(6), 349-357. https://doi.org/10.11648/j.jps.20150306.19

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Maholy Pricille Ratsimiebo; David Ramanitrahasimbola; Clara Fredeline Rajemiarimoelisoa; Zoarilala Rinah Razafindrakoto; Hanitra Ranjana Randrianarivo, et al. Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar. J. Plant Sci. 2015, 3(6), 349-357. doi: 10.11648/j.jps.20150306.19

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Maholy Pricille Ratsimiebo, David Ramanitrahasimbola, Clara Fredeline Rajemiarimoelisoa, Zoarilala Rinah Razafindrakoto, Hanitra Ranjana Randrianarivo, et al. Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar. J Plant Sci. 2015;3(6):349-357. doi: 10.11648/j.jps.20150306.19

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@article{10.11648/j.jps.20150306.19,
  author = {Maholy Pricille Ratsimiebo and David Ramanitrahasimbola and Clara Fredeline Rajemiarimoelisoa and Zoarilala Rinah Razafindrakoto and Hanitra Ranjana Randrianarivo and Danielle Aurore Doll Rakoto and Victor Louis Jeannoda},
  title = {Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {6},
  pages = {349-357},
  doi = {10.11648/j.jps.20150306.19},
  url = {https://doi.org/10.11648/j.jps.20150306.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150306.19},
  abstract = {The present work aimed to assess the leaf toxicity of Pittosporum ochrosiaefolium Bojer, a well-known medicinal plant endemic to Madagascar. Leaf methanolic extract (LME), obtained after successive extractions by hexan and methanol, was tested in vivo on warm and cold-blooded animals and in vitro on isolated atria of guinea-pig. LME was toxic to mice with a LD50 of about 46.69 mg/kg of body weight by intraperitoneal route. It induced mainly nervous disorders (body fasciculation, clonic convulsions), respiratory troubles (reduction of respiration frequency and cyanosis) and diarrheas. By intraperitoneal route, LME (46.69 mg/kg) caused histopathological lesions in lungs, liver, kidneys, small and large intestines but had no effects on brain, heart and stomach. Vascular congestion, inflammatory infiltrates, edema and necrosis were frequently observed. LME had a positive inotropic effect but no significant chronotropic one on isolated atria. It did not alter renal and hepatic functions at 21.24 mg/kg. It was highly toxic to the frog Ptychadena mascareniensis (LC50 of 13.51 µg/mL) and the fish Cyprinus carpio (LC50 of 8.2 µg/mL). It was also toxic to mosquito larvae Culex quinquefasciatus and Aedes albopictus with LC50 of 720 ppm and 910 ppm respectively. Different chemical compound groups were found in LME but only saponins proved to be toxic. Under certain conditions, P. ochrosiaefolium might be exploited as source of pesticides or therapeutic molecules.},
 year = {2015}
}

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TY - JOUR
T1 - Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar
AU - Maholy Pricille Ratsimiebo
AU - David Ramanitrahasimbola
AU - Clara Fredeline Rajemiarimoelisoa
AU - Zoarilala Rinah Razafindrakoto
AU - Hanitra Ranjana Randrianarivo
AU - Danielle Aurore Doll Rakoto
AU - Victor Louis Jeannoda
Y1 - 2015/12/10
PY - 2015
N1 - https://doi.org/10.11648/j.jps.20150306.19
DO - 10.11648/j.jps.20150306.19
T2 - Journal of Plant Sciences
JF - Journal of Plant Sciences
JO - Journal of Plant Sciences
SP - 349
EP - 357
PB - Science Publishing Group
SN - 2331-0731
UR - https://doi.org/10.11648/j.jps.20150306.19
AB - The present work aimed to assess the leaf toxicity of Pittosporum ochrosiaefolium Bojer, a well-known medicinal plant endemic to Madagascar. Leaf methanolic extract (LME), obtained after successive extractions by hexan and methanol, was tested in vivo on warm and cold-blooded animals and in vitro on isolated atria of guinea-pig. LME was toxic to mice with a LD50 of about 46.69 mg/kg of body weight by intraperitoneal route. It induced mainly nervous disorders (body fasciculation, clonic convulsions), respiratory troubles (reduction of respiration frequency and cyanosis) and diarrheas. By intraperitoneal route, LME (46.69 mg/kg) caused histopathological lesions in lungs, liver, kidneys, small and large intestines but had no effects on brain, heart and stomach. Vascular congestion, inflammatory infiltrates, edema and necrosis were frequently observed. LME had a positive inotropic effect but no significant chronotropic one on isolated atria. It did not alter renal and hepatic functions at 21.24 mg/kg. It was highly toxic to the frog Ptychadena mascareniensis (LC50 of 13.51 µg/mL) and the fish Cyprinus carpio (LC50 of 8.2 µg/mL). It was also toxic to mosquito larvae Culex quinquefasciatus and Aedes albopictus with LC50 of 720 ppm and 910 ppm respectively. Different chemical compound groups were found in LME but only saponins proved to be toxic. Under certain conditions, P. ochrosiaefolium might be exploited as source of pesticides or therapeutic molecules.
VL - 3
IS - 6
ER -

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Author Information

Maholy Pricille Ratsimiebo

Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
David Ramanitrahasimbola

Department of Pharmacy, Faculty of Medicine, University of Antananarivo, Antananarivo, Madagascar
Clara Fredeline Rajemiarimoelisoa

Department of Pharmacy, Faculty of Medicine, University of Antananarivo, Antananarivo, Madagascar
Zoarilala Rinah Razafindrakoto

Malagasy Institute for Applied Research (IMRA), Antananarivo, Madagascar
Hanitra Ranjana Randrianarivo

Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Danielle Aurore Doll Rakoto

Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar
Victor Louis Jeannoda

Laboratory of Applied Biochemistry to Medical Sciences, Fundamental and Applied Biochemistry Department, Faculty of Sciences, University of Antananarivo, Antananarivo, Madagascar

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Maholy Pricille Ratsimiebo, David Ramanitrahasimbola, Clara Fredeline Rajemiarimoelisoa, Zoarilala Rinah Razafindrakoto, Hanitra Ranjana Randrianarivo, et al. (2015). Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar. Journal of Plant Sciences, 3(6), 349-357. https://doi.org/10.11648/j.jps.20150306.19

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Maholy Pricille Ratsimiebo; David Ramanitrahasimbola; Clara Fredeline Rajemiarimoelisoa; Zoarilala Rinah Razafindrakoto; Hanitra Ranjana Randrianarivo, et al. Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar. J. Plant Sci. 2015, 3(6), 349-357. doi: 10.11648/j.jps.20150306.19

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AMA Style

Maholy Pricille Ratsimiebo, David Ramanitrahasimbola, Clara Fredeline Rajemiarimoelisoa, Zoarilala Rinah Razafindrakoto, Hanitra Ranjana Randrianarivo, et al. Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar. J Plant Sci. 2015;3(6):349-357. doi: 10.11648/j.jps.20150306.19

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@article{10.11648/j.jps.20150306.19,
  author = {Maholy Pricille Ratsimiebo and David Ramanitrahasimbola and Clara Fredeline Rajemiarimoelisoa and Zoarilala Rinah Razafindrakoto and Hanitra Ranjana Randrianarivo and Danielle Aurore Doll Rakoto and Victor Louis Jeannoda},
  title = {Toxicity Study of Pittosporum ochrosiaefolium Bojer (Pittosporaceae) a Medicinal Plant of Madagascar},
  journal = {Journal of Plant Sciences},
  volume = {3},
  number = {6},
  pages = {349-357},
  doi = {10.11648/j.jps.20150306.19},
  url = {https://doi.org/10.11648/j.jps.20150306.19},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jps.20150306.19},
  abstract = {The present work aimed to assess the leaf toxicity of Pittosporum ochrosiaefolium Bojer, a well-known medicinal plant endemic to Madagascar. Leaf methanolic extract (LME), obtained after successive extractions by hexan and methanol, was tested in vivo on warm and cold-blooded animals and in vitro on isolated atria of guinea-pig. LME was toxic to mice with a LD50 of about 46.69 mg/kg of body weight by intraperitoneal route. It induced mainly nervous disorders (body fasciculation, clonic convulsions), respiratory troubles (reduction of respiration frequency and cyanosis) and diarrheas. By intraperitoneal route, LME (46.69 mg/kg) caused histopathological lesions in lungs, liver, kidneys, small and large intestines but had no effects on brain, heart and stomach. Vascular congestion, inflammatory infiltrates, edema and necrosis were frequently observed. LME had a positive inotropic effect but no significant chronotropic one on isolated atria. It did not alter renal and hepatic functions at 21.24 mg/kg. It was highly toxic to the frog Ptychadena mascareniensis (LC50 of 13.51 µg/mL) and the fish Cyprinus carpio (LC50 of 8.2 µg/mL). It was also toxic to mosquito larvae Culex quinquefasciatus and Aedes albopictus with LC50 of 720 ppm and 910 ppm respectively. Different chemical compound groups were found in LME but only saponins proved to be toxic. Under certain conditions, P. ochrosiaefolium might be exploited as source of pesticides or therapeutic molecules.},
 year = {2015}
}

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