Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium

Yanlin Zhang; Jiandong Wu; Lulu Ren; Guning Wang; Lihong Yuan; Zhihui Zou

doi:doi:10.11648/j.jher.20190503.13

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Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium

Yanlin Zhang, Jiandong Wu, Lulu Ren, Guning Wang, Lihong Yuan, Zhihui Zou

Published in Journal of Health and Environmental Research (Volume 5, Issue 3)

Received: 18 November 2018 Accepted: 13 December 2018 Published: 11 October 2019

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Abstract

Backgrounds Some studies have reported that different dosage of cadmium (Cd) had a biphasic effect on cells viability, Cd at a dose of more than 20 μM can induce excessive apoptosis which past studies were mainly concentrated on while there has been few reports on a relatively lower dose of Cd causing abnormal proliferation. Kidney is the most sensitive target organ of Cd, however, the mechanism how Cd at a relatively lower dose affects the proliferation of renal tubular epithelial cells (RTEC) is not yet clear at present. Objectives To explore the toxic effect of Cd at a relatively lower dose on RTEC and study the mechanism how the TGF-β1-mediated Wnt/β-catenin pathways involved in EMT-like transformation induced by Cadmium. Methods The RTEC were isolated by mixed collagenase digestion methods and the effect of Cd at different dose on cell viability was detected by MTT assay. The effect of Cd at Hormesis zone dose on the expression of c-myc, cyclinD1, α-SMA, TGF-β1, Wnt and β-catenin were determined by qRT-PCR. Results When exposed for 24h-72h, Cd at a dose of 2.5 μM had maximum proliferation promoting effect. Cd at Hormesis zone dose could up-regulate the expression of cell cycle and proliferation key regulators c-myc and cyclinD1 in an obvious time-dependent manner. Cd at Hormesis zone dose could significantly promote α-SMA, TGF-β1, Wnt and β-catenin mRNA expression. Conclusions Cd at Hormesis zone dose could induce RTEC significant abnormal proliferation and EMT-like transformation. The activation of TGF-β1-mediated Wnt/β-catenin pathways might play a key role in EMT-like transformation induced by Cd at Hormesis zone dose.

Published in	Journal of Health and Environmental Research (Volume 5, Issue 3)
DOI	10.11648/j.jher.20190503.13
Page(s)	87-94
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Wnt/β-catenin, TGF-β1, EMT-like Transformation, Cadmium

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APA Style

Yanlin Zhang, Jiandong Wu, Lulu Ren, Guning Wang, Lihong Yuan, et al. (2019). Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium. Journal of Health and Environmental Research, 5(3), 87-94. https://doi.org/10.11648/j.jher.20190503.13

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ACS Style

Yanlin Zhang; Jiandong Wu; Lulu Ren; Guning Wang; Lihong Yuan, et al. Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium. J. Health Environ. Res. 2019, 5(3), 87-94. doi: 10.11648/j.jher.20190503.13

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AMA Style

Yanlin Zhang, Jiandong Wu, Lulu Ren, Guning Wang, Lihong Yuan, et al. Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium. J Health Environ Res. 2019;5(3):87-94. doi: 10.11648/j.jher.20190503.13

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@article{10.11648/j.jher.20190503.13,
  author = {Yanlin Zhang and Jiandong Wu and Lulu Ren and Guning Wang and Lihong Yuan and Zhihui Zou},
  title = {Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium},
  journal = {Journal of Health and Environmental Research},
  volume = {5},
  number = {3},
  pages = {87-94},
  doi = {10.11648/j.jher.20190503.13},
  url = {https://doi.org/10.11648/j.jher.20190503.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20190503.13},
  abstract = {Backgrounds Some studies have reported that different dosage of cadmium (Cd) had a biphasic effect on cells viability, Cd at a dose of more than 20 μM can induce excessive apoptosis which past studies were mainly concentrated on while there has been few reports on a relatively lower dose of Cd causing abnormal proliferation. Kidney is the most sensitive target organ of Cd, however, the mechanism how Cd at a relatively lower dose affects the proliferation of renal tubular epithelial cells (RTEC) is not yet clear at present. Objectives To explore the toxic effect of Cd at a relatively lower dose on RTEC and study the mechanism how the TGF-β1-mediated Wnt/β-catenin pathways involved in EMT-like transformation induced by Cadmium. Methods The RTEC were isolated by mixed collagenase digestion methods and the effect of Cd at different dose on cell viability was detected by MTT assay. The effect of Cd at Hormesis zone dose on the expression of c-myc, cyclinD1, α-SMA, TGF-β1, Wnt and β-catenin were determined by qRT-PCR. Results When exposed for 24h-72h, Cd at a dose of 2.5 μM had maximum proliferation promoting effect. Cd at Hormesis zone dose could up-regulate the expression of cell cycle and proliferation key regulators c-myc and cyclinD1 in an obvious time-dependent manner. Cd at Hormesis zone dose could significantly promote α-SMA, TGF-β1, Wnt and β-catenin mRNA expression. Conclusions Cd at Hormesis zone dose could induce RTEC significant abnormal proliferation and EMT-like transformation. The activation of TGF-β1-mediated Wnt/β-catenin pathways might play a key role in EMT-like transformation induced by Cd at Hormesis zone dose.},
 year = {2019}
}

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TY  - JOUR
T1  - Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium
AU  - Yanlin Zhang
AU  - Jiandong Wu
AU  - Lulu Ren
AU  - Guning Wang
AU  - Lihong Yuan
AU  - Zhihui Zou
Y1  - 2019/10/11
PY  - 2019
N1  - https://doi.org/10.11648/j.jher.20190503.13
DO  - 10.11648/j.jher.20190503.13
T2  - Journal of Health and Environmental Research
JF  - Journal of Health and Environmental Research
JO  - Journal of Health and Environmental Research
SP  - 87
EP  - 94
PB  - Science Publishing Group
SN  - 2472-3592
UR  - https://doi.org/10.11648/j.jher.20190503.13
AB  - Backgrounds Some studies have reported that different dosage of cadmium (Cd) had a biphasic effect on cells viability, Cd at a dose of more than 20 μM can induce excessive apoptosis which past studies were mainly concentrated on while there has been few reports on a relatively lower dose of Cd causing abnormal proliferation. Kidney is the most sensitive target organ of Cd, however, the mechanism how Cd at a relatively lower dose affects the proliferation of renal tubular epithelial cells (RTEC) is not yet clear at present. Objectives To explore the toxic effect of Cd at a relatively lower dose on RTEC and study the mechanism how the TGF-β1-mediated Wnt/β-catenin pathways involved in EMT-like transformation induced by Cadmium. Methods The RTEC were isolated by mixed collagenase digestion methods and the effect of Cd at different dose on cell viability was detected by MTT assay. The effect of Cd at Hormesis zone dose on the expression of c-myc, cyclinD1, α-SMA, TGF-β1, Wnt and β-catenin were determined by qRT-PCR. Results When exposed for 24h-72h, Cd at a dose of 2.5 μM had maximum proliferation promoting effect. Cd at Hormesis zone dose could up-regulate the expression of cell cycle and proliferation key regulators c-myc and cyclinD1 in an obvious time-dependent manner. Cd at Hormesis zone dose could significantly promote α-SMA, TGF-β1, Wnt and β-catenin mRNA expression. Conclusions Cd at Hormesis zone dose could induce RTEC significant abnormal proliferation and EMT-like transformation. The activation of TGF-β1-mediated Wnt/β-catenin pathways might play a key role in EMT-like transformation induced by Cd at Hormesis zone dose.
VL  - 5
IS  - 3
ER  -

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Author Information

Yanlin Zhang

Department of Environmental Monitoring, Guangdong Vocational College of Environmental Protection Engineering, Foshan, China
Jiandong Wu

Department of Environmental Monitoring, Guangdong Vocational College of Environmental Protection Engineering, Foshan, China
Lulu Ren

Department of Environmental Monitoring, Guangdong Vocational College of Environmental Protection Engineering, Foshan, China
Guning Wang

Department of Environmental Monitoring, Guangdong Vocational College of Environmental Protection Engineering, Foshan, China
Lihong Yuan

Department of Environmental Monitoring, Guangdong Vocational College of Environmental Protection Engineering, Foshan, China
Zhihui Zou

School of Public Health, Guangdong Pharmaceutical University, Guangzhou, China

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APA Style

Yanlin Zhang, Jiandong Wu, Lulu Ren, Guning Wang, Lihong Yuan, et al. (2019). Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium. Journal of Health and Environmental Research, 5(3), 87-94. https://doi.org/10.11648/j.jher.20190503.13

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ACS Style

Yanlin Zhang; Jiandong Wu; Lulu Ren; Guning Wang; Lihong Yuan, et al. Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium. J. Health Environ. Res. 2019, 5(3), 87-94. doi: 10.11648/j.jher.20190503.13

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AMA Style

Yanlin Zhang, Jiandong Wu, Lulu Ren, Guning Wang, Lihong Yuan, et al. Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium. J Health Environ Res. 2019;5(3):87-94. doi: 10.11648/j.jher.20190503.13

Copy | Download

@article{10.11648/j.jher.20190503.13,
  author = {Yanlin Zhang and Jiandong Wu and Lulu Ren and Guning Wang and Lihong Yuan and Zhihui Zou},
  title = {Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium},
  journal = {Journal of Health and Environmental Research},
  volume = {5},
  number = {3},
  pages = {87-94},
  doi = {10.11648/j.jher.20190503.13},
  url = {https://doi.org/10.11648/j.jher.20190503.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jher.20190503.13},
  abstract = {Backgrounds Some studies have reported that different dosage of cadmium (Cd) had a biphasic effect on cells viability, Cd at a dose of more than 20 μM can induce excessive apoptosis which past studies were mainly concentrated on while there has been few reports on a relatively lower dose of Cd causing abnormal proliferation. Kidney is the most sensitive target organ of Cd, however, the mechanism how Cd at a relatively lower dose affects the proliferation of renal tubular epithelial cells (RTEC) is not yet clear at present. Objectives To explore the toxic effect of Cd at a relatively lower dose on RTEC and study the mechanism how the TGF-β1-mediated Wnt/β-catenin pathways involved in EMT-like transformation induced by Cadmium. Methods The RTEC were isolated by mixed collagenase digestion methods and the effect of Cd at different dose on cell viability was detected by MTT assay. The effect of Cd at Hormesis zone dose on the expression of c-myc, cyclinD1, α-SMA, TGF-β1, Wnt and β-catenin were determined by qRT-PCR. Results When exposed for 24h-72h, Cd at a dose of 2.5 μM had maximum proliferation promoting effect. Cd at Hormesis zone dose could up-regulate the expression of cell cycle and proliferation key regulators c-myc and cyclinD1 in an obvious time-dependent manner. Cd at Hormesis zone dose could significantly promote α-SMA, TGF-β1, Wnt and β-catenin mRNA expression. Conclusions Cd at Hormesis zone dose could induce RTEC significant abnormal proliferation and EMT-like transformation. The activation of TGF-β1-mediated Wnt/β-catenin pathways might play a key role in EMT-like transformation induced by Cd at Hormesis zone dose.},
 year = {2019}
}

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TY  - JOUR
T1  - Regulation of TGF-β1-Mediated Wnt/β-catenin Pathways Plays an Important Role in EMT-like Transformation Induced by Cadmium
AU  - Yanlin Zhang
AU  - Jiandong Wu
AU  - Lulu Ren
AU  - Guning Wang
AU  - Lihong Yuan
AU  - Zhihui Zou
Y1  - 2019/10/11
PY  - 2019
N1  - https://doi.org/10.11648/j.jher.20190503.13
DO  - 10.11648/j.jher.20190503.13
T2  - Journal of Health and Environmental Research
JF  - Journal of Health and Environmental Research
JO  - Journal of Health and Environmental Research
SP  - 87
EP  - 94
PB  - Science Publishing Group
SN  - 2472-3592
UR  - https://doi.org/10.11648/j.jher.20190503.13
AB  - Backgrounds Some studies have reported that different dosage of cadmium (Cd) had a biphasic effect on cells viability, Cd at a dose of more than 20 μM can induce excessive apoptosis which past studies were mainly concentrated on while there has been few reports on a relatively lower dose of Cd causing abnormal proliferation. Kidney is the most sensitive target organ of Cd, however, the mechanism how Cd at a relatively lower dose affects the proliferation of renal tubular epithelial cells (RTEC) is not yet clear at present. Objectives To explore the toxic effect of Cd at a relatively lower dose on RTEC and study the mechanism how the TGF-β1-mediated Wnt/β-catenin pathways involved in EMT-like transformation induced by Cadmium. Methods The RTEC were isolated by mixed collagenase digestion methods and the effect of Cd at different dose on cell viability was detected by MTT assay. The effect of Cd at Hormesis zone dose on the expression of c-myc, cyclinD1, α-SMA, TGF-β1, Wnt and β-catenin were determined by qRT-PCR. Results When exposed for 24h-72h, Cd at a dose of 2.5 μM had maximum proliferation promoting effect. Cd at Hormesis zone dose could up-regulate the expression of cell cycle and proliferation key regulators c-myc and cyclinD1 in an obvious time-dependent manner. Cd at Hormesis zone dose could significantly promote α-SMA, TGF-β1, Wnt and β-catenin mRNA expression. Conclusions Cd at Hormesis zone dose could induce RTEC significant abnormal proliferation and EMT-like transformation. The activation of TGF-β1-mediated Wnt/β-catenin pathways might play a key role in EMT-like transformation induced by Cd at Hormesis zone dose.
VL  - 5
IS  - 3
ER  -

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