Effectiveness of <i>Coriandrum sativum</i> Extract as Antimicrobial by Applying on Six Bacterial Strains

Faroug Bakheit Mohamed Ahmed; Abd El-Mohymen Jaber Alla

doi:doi:10.11648/j.jdmp.20190506.13

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Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains

Faroug Bakheit Mohamed Ahmed, Abd El-Mohymen Jaber Alla

Published in Journal of Diseases and Medicinal Plants (Volume 5, Issue 6)

Received: 23 September 2019 Accepted: 27 November 2019 Published: 7 December 2019

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Abstract

The study aimed to detect the antibacterial activity of coriander extract via investigation of six types of bacteria; three gram-positive reference strain were (Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), whilst three gram-negative reference strains were (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311 and Pseudomonas aeruginosa ATCC 27853). All bacterial strains were stored in Brain Heart Infusion (BHI) broth with 20% (v/v) glycerol at 280°C. Prior to susceptibility testing, each strain was inoculated on BHI agar to ensure optimal growth and purity plating suitable. Four different concentrations (12.5%, 25%, 50% and 100%) of the oil were dissolved in dimethyl sulfoxide (DMSO) in triplicates using nutrient agar medium. In general, our study revealed that the coriander oil appeared higher influence on the positive gram strain rather than negative gram strain; this might be due to the gram positive strains are more sensitive and potential of hydrophobic essential oils to disrupt the bacterial cell membrane. The results showed that all tested concentrations of coriander oil as antibacterial activity against gram positive and gram negative bacteria; Salmonella typhimurium and Enterococcus fecalis respectively were appeared resistance at the concentrations 12.5%. In addition the concentration 100% inflicted the highest antibacterial activities. The most susceptible bacteria strains was Staphylococcus aureus with highest inhibition zone values (33mm) at concentration 25% and 100% (40mm).

Published in	Journal of Diseases and Medicinal Plants (Volume 5, Issue 6)
DOI	10.11648/j.jdmp.20190506.13
Page(s)	103-106
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Coriander, Extract, Antibacterial, Strain and DMSO

References

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[2]	Ravi R. M. and Prakash K. K. (2007). Bhat aroma characterization of coriander (Coriandrum sativum L.) oil samples. Eur. Food Res. Technol, 225 pp. 367–374.
[3]	Raal A., Arak E. and Orav A. (2004). Chemical composition of coriander seed essential oil and their conformity with EP standards Agraarteadus, 15: 234–239.
[4]	Coskuner Y. and Karababa E. (2007). Physical properties of coriander seeds (Coriandrum sativum L.) J. Food Eng., 80, pp. 408-416.
[5]	Mahendra P. and Bisht S. (2011). Coriandrum sativum: a daily use spice with great medicinal effect Pharmacogn J, 3: 84–88.
[6]	Keskin S. and Toroglu S. (2011). Studies on antimicrobial activities of solvent extracts of different spices J Environ Biol, 32, pp. 251–256.
[7]	Shahwar M. K., El- Ghorab A. H., Anjum F. M., Butt M. S. Hussain S. and Nadeem M. (2012). Characterization of coriander (Coriandrum sativum L.) seeds and leaves: volatile and non volatile extracts. Int J Food Prop, 15, pp. 736–747.
[8]	Shibamoto K., Mochizuki M. and Kusuhara, M. (2010). Aroma Therapy in Anti-AgingMedicine. Anti-Aging Medicine, Vol. 7, pp. 55-59.
[9]	Porto C., Decorti D. and Kikic I. (2009). Flavour compounds of Lavandula angustifolia L. to use in food manufacturing: Comparison of three different extraction methods. Food Chemistry, Vol. 112, pp. 1072-1078.
[10]	Ates D. A. and Erdogrul O. T. (2003). Antimicrobial activities of various medicinal and commercial plant extracts Turk J Biol, 27, pp. 157–162.
[11]	Pasqua R., Betts G., Hoskins N., Edwards M., Ercolini D. and Mauriello G. (2007). Membrane toxicity of antimicrobial compounds from essential oils. J. Agric. Food Chem., Vol. 55, pp. 4863-4870.
[12]	Darughe F. Barzegar M. and Sahari M. A. (2012). Antioxidant and antifungal activity of coriander (Coriandrum sativum L.) essential oil in cake Int Food Res; J, 19: 1253–1260.
[13]	Teshale C. Hussien J. and Jemal A. (2013). Antimicrobial activity of the extracts of selected Ethiopian aromatic medicinal plants. Spatula DD, 3: 175–180.
[14]	Wangesteen H., Samuelsen A. B. and Malterud K. E. (2004). Food Chem., 88: 293.

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Faroug Bakheit Mohamed Ahmed, Abd El-Mohymen Jaber Alla. (2019). Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains. Journal of Diseases and Medicinal Plants, 5(6), 103-106. https://doi.org/10.11648/j.jdmp.20190506.13

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ACS Style

Faroug Bakheit Mohamed Ahmed; Abd El-Mohymen Jaber Alla. Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains. J. Dis. Med. Plants 2019, 5(6), 103-106. doi: 10.11648/j.jdmp.20190506.13

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AMA Style

Faroug Bakheit Mohamed Ahmed, Abd El-Mohymen Jaber Alla. Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains. J Dis Med Plants. 2019;5(6):103-106. doi: 10.11648/j.jdmp.20190506.13

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@article{10.11648/j.jdmp.20190506.13,
  author = {Faroug Bakheit Mohamed Ahmed and Abd El-Mohymen Jaber Alla},
  title = {Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {5},
  number = {6},
  pages = {103-106},
  doi = {10.11648/j.jdmp.20190506.13},
  url = {https://doi.org/10.11648/j.jdmp.20190506.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20190506.13},
  abstract = {The study aimed to detect the antibacterial activity of coriander extract via investigation of six types of bacteria; three gram-positive reference strain were (Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), whilst three gram-negative reference strains were (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311 and Pseudomonas aeruginosa ATCC 27853). All bacterial strains were stored in Brain Heart Infusion (BHI) broth with 20% (v/v) glycerol at 280°C. Prior to susceptibility testing, each strain was inoculated on BHI agar to ensure optimal growth and purity plating suitable. Four different concentrations (12.5%, 25%, 50% and 100%) of the oil were dissolved in dimethyl sulfoxide (DMSO) in triplicates using nutrient agar medium. In general, our study revealed that the coriander oil appeared higher influence on the positive gram strain rather than negative gram strain; this might be due to the gram positive strains are more sensitive and potential of hydrophobic essential oils to disrupt the bacterial cell membrane. The results showed that all tested concentrations of coriander oil as antibacterial activity against gram positive and gram negative bacteria; Salmonella typhimurium and Enterococcus fecalis respectively were appeared resistance at the concentrations 12.5%. In addition the concentration 100% inflicted the highest antibacterial activities. The most susceptible bacteria strains was Staphylococcus aureus with highest inhibition zone values (33mm) at concentration 25% and 100% (40mm).},
 year = {2019}
}

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TY  - JOUR
T1  - Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains
AU  - Faroug Bakheit Mohamed Ahmed
AU  - Abd El-Mohymen Jaber Alla
Y1  - 2019/12/07
PY  - 2019
N1  - https://doi.org/10.11648/j.jdmp.20190506.13
DO  - 10.11648/j.jdmp.20190506.13
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 103
EP  - 106
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20190506.13
AB  - The study aimed to detect the antibacterial activity of coriander extract via investigation of six types of bacteria; three gram-positive reference strain were (Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), whilst three gram-negative reference strains were (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311 and Pseudomonas aeruginosa ATCC 27853). All bacterial strains were stored in Brain Heart Infusion (BHI) broth with 20% (v/v) glycerol at 280°C. Prior to susceptibility testing, each strain was inoculated on BHI agar to ensure optimal growth and purity plating suitable. Four different concentrations (12.5%, 25%, 50% and 100%) of the oil were dissolved in dimethyl sulfoxide (DMSO) in triplicates using nutrient agar medium. In general, our study revealed that the coriander oil appeared higher influence on the positive gram strain rather than negative gram strain; this might be due to the gram positive strains are more sensitive and potential of hydrophobic essential oils to disrupt the bacterial cell membrane. The results showed that all tested concentrations of coriander oil as antibacterial activity against gram positive and gram negative bacteria; Salmonella typhimurium and Enterococcus fecalis respectively were appeared resistance at the concentrations 12.5%. In addition the concentration 100% inflicted the highest antibacterial activities. The most susceptible bacteria strains was Staphylococcus aureus with highest inhibition zone values (33mm) at concentration 25% and 100% (40mm).
VL  - 5
IS  - 6
ER  -

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Author Information

Faroug Bakheit Mohamed Ahmed

Faculty of Science and Technology, Shendi University, Shendi, Sudan
Abd El-Mohymen Jaber Alla

Faculty of Science and Technology, Shendi University, Shendi, Sudan

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Plain Text BibTeX RIS

APA Style

Faroug Bakheit Mohamed Ahmed, Abd El-Mohymen Jaber Alla. (2019). Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains. Journal of Diseases and Medicinal Plants, 5(6), 103-106. https://doi.org/10.11648/j.jdmp.20190506.13

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ACS Style

Faroug Bakheit Mohamed Ahmed; Abd El-Mohymen Jaber Alla. Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains. J. Dis. Med. Plants 2019, 5(6), 103-106. doi: 10.11648/j.jdmp.20190506.13

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AMA Style

Faroug Bakheit Mohamed Ahmed, Abd El-Mohymen Jaber Alla. Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains. J Dis Med Plants. 2019;5(6):103-106. doi: 10.11648/j.jdmp.20190506.13

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@article{10.11648/j.jdmp.20190506.13,
  author = {Faroug Bakheit Mohamed Ahmed and Abd El-Mohymen Jaber Alla},
  title = {Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains},
  journal = {Journal of Diseases and Medicinal Plants},
  volume = {5},
  number = {6},
  pages = {103-106},
  doi = {10.11648/j.jdmp.20190506.13},
  url = {https://doi.org/10.11648/j.jdmp.20190506.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.jdmp.20190506.13},
  abstract = {The study aimed to detect the antibacterial activity of coriander extract via investigation of six types of bacteria; three gram-positive reference strain were (Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), whilst three gram-negative reference strains were (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311 and Pseudomonas aeruginosa ATCC 27853). All bacterial strains were stored in Brain Heart Infusion (BHI) broth with 20% (v/v) glycerol at 280°C. Prior to susceptibility testing, each strain was inoculated on BHI agar to ensure optimal growth and purity plating suitable. Four different concentrations (12.5%, 25%, 50% and 100%) of the oil were dissolved in dimethyl sulfoxide (DMSO) in triplicates using nutrient agar medium. In general, our study revealed that the coriander oil appeared higher influence on the positive gram strain rather than negative gram strain; this might be due to the gram positive strains are more sensitive and potential of hydrophobic essential oils to disrupt the bacterial cell membrane. The results showed that all tested concentrations of coriander oil as antibacterial activity against gram positive and gram negative bacteria; Salmonella typhimurium and Enterococcus fecalis respectively were appeared resistance at the concentrations 12.5%. In addition the concentration 100% inflicted the highest antibacterial activities. The most susceptible bacteria strains was Staphylococcus aureus with highest inhibition zone values (33mm) at concentration 25% and 100% (40mm).},
 year = {2019}
}

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TY  - JOUR
T1  - Effectiveness of Coriandrum sativum Extract as Antimicrobial by Applying on Six Bacterial Strains
AU  - Faroug Bakheit Mohamed Ahmed
AU  - Abd El-Mohymen Jaber Alla
Y1  - 2019/12/07
PY  - 2019
N1  - https://doi.org/10.11648/j.jdmp.20190506.13
DO  - 10.11648/j.jdmp.20190506.13
T2  - Journal of Diseases and Medicinal Plants
JF  - Journal of Diseases and Medicinal Plants
JO  - Journal of Diseases and Medicinal Plants
SP  - 103
EP  - 106
PB  - Science Publishing Group
SN  - 2469-8210
UR  - https://doi.org/10.11648/j.jdmp.20190506.13
AB  - The study aimed to detect the antibacterial activity of coriander extract via investigation of six types of bacteria; three gram-positive reference strain were (Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923 and Enterococcus faecalis ATCC 29212), whilst three gram-negative reference strains were (Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 13311 and Pseudomonas aeruginosa ATCC 27853). All bacterial strains were stored in Brain Heart Infusion (BHI) broth with 20% (v/v) glycerol at 280°C. Prior to susceptibility testing, each strain was inoculated on BHI agar to ensure optimal growth and purity plating suitable. Four different concentrations (12.5%, 25%, 50% and 100%) of the oil were dissolved in dimethyl sulfoxide (DMSO) in triplicates using nutrient agar medium. In general, our study revealed that the coriander oil appeared higher influence on the positive gram strain rather than negative gram strain; this might be due to the gram positive strains are more sensitive and potential of hydrophobic essential oils to disrupt the bacterial cell membrane. The results showed that all tested concentrations of coriander oil as antibacterial activity against gram positive and gram negative bacteria; Salmonella typhimurium and Enterococcus fecalis respectively were appeared resistance at the concentrations 12.5%. In addition the concentration 100% inflicted the highest antibacterial activities. The most susceptible bacteria strains was Staphylococcus aureus with highest inhibition zone values (33mm) at concentration 25% and 100% (40mm).
VL  - 5
IS  - 6
ER  -

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