Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon

Maffo Tazoho Ghislain; Agbor Esther Etengeneng; Mekoudjou Nouwou Hermine Sonia; Kengne Sikati Christelle Noelle; Gouado Inocent

doi:doi:10.11648/j.ijnfs.20140304.14

Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon

Maffo Tazoho Ghislain, Agbor Esther Etengeneng, Mekoudjou Nouwou Hermine Sonia, Kengne Sikati Christelle Noelle, Gouado Inocent

Published in International Journal of Nutrition and Food Sciences (Volume 3, Issue 4)

Received: 14 May 2014 Accepted: 7 June 2014 Published: 30 June 2014

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Abstract

The chemical composition and protein quality of raw, boiled, toasted and soak-toasted roselle seeds harvested in the North (area I) and West (area II) regions of Cameroon were investigated in this study. To conduct the growth and nitrogen balance study, sixty albinos wistar rats of 21 to 25 days separated into ten groups of six rats/group were used and fed for fourteen days with 10% (w/w) isoproteinous experimental diets. These groups were Protein free diet (D0), egg white-standard reference protein diet (D1) and raw (D2I, D2II), boiled (D3I, D3II), toasted (D4I, D4II) and soak-toasted (D5II, D6II) roselle seed diets. Transaminases, serum creatinine, haematocrit, lipid parameters were also assessed in the rats. Results showed that crude protein, lipid, fiber and ash content of seed samples ranged respectively from 22 to 26, 18 to 22, 18 to 23 and 4.3 to 6.4%. The contents of Ca, Mg, K, Na, P, Fe and Zn were ranged from 1054 to 1920, 1670 to 2083, 26.45 to 272.7, 14 to 22, 10.58 to 90.78, 137.3 to 169.22 and 10.13 to 70.11 mg/kg respectively. Food intake was higher (P<0.05) for groups D1, D3I and D3II than for others groups. The rats fed with reference diet followed by boiled seed diets had the highest (P<0.05) weight gained, protein efficiency ratio (PER), net protein ratio (NPR), feed efficiency (FE), efficiency of food utilization (EFU) and true digestibility (TD). Aspartate aminotransferase and alanine aminotranferase were highest (p<0.05) in the rats fed raw roselle seeds. Compared to reference diet, the total cholesterol (TC), triglycerides (TG) and LDL-C of rats fed with diet D5II decreased while the HDL-C did not show any significant changes in these rats (p˃0.05). These findings show that roselle seeds cultivated in Cameroon have important amount of macronutrients and minerals. Proteins of roselle seeds are of good quality especially from the boiled seeds and are not influenced by the agro ecological region. Consumption of roselle seeds might be beneficial for cardio protective effect and no toxicity was observed as far as transaminases are concerned except that for the raw seeds which showed significant hepatotoxicity.

Published in	International Journal of Nutrition and Food Sciences (Volume 3, Issue 4)
DOI	10.11648/j.ijnfs.20140304.14
Page(s)	251-258
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Roselle Seeds, Chemical Composition, Protein Quality, Transaminases, Lipid Profile, Rats

References

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[22]	Modi A. T., 2007. Growth temperature and plant age influence on nutritional on nutritional quality of amaranthus leaves and seed germination capacity. Crop Science, School of Agricultural Sciences and Agro business, University of KwaZulu-Natal, private bag X01, Scottsville 3201, South Africa.
[23]	Mohamadou B. A., C. M. F. Mbofung and D. Thouvenot, 2007. Functional potential of a product from traditional biotechnology: antioxidant and probiotic potential of Mbuja, produced by fermentation of Hibiscus sabdariffa seeds Cameroon. J. Food Technol., 5 (2): 164-168.
[24]	Parkouda C., B. Diawara and L. I. I. Ouoda, 2008. Technology and physic-chemical characteristics of Bikalga, alkaline fermented seeds of Hibiscus sabdariffa. Afr. J. Biotechnol., 7(7): 916-922.
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[31]	Yacoub A. A. and A. A. Abdalla, 2007. Effect of domestic processing methods on chemical, in vitro digestibility of protein and starch and functional properties of Bambara groundnut (Voandzeia subterranean) seed. J. Agric. Biol. Sci., 3: 24-34.

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Maffo Tazoho Ghislain, Agbor Esther Etengeneng, Mekoudjou Nouwou Hermine Sonia, Kengne Sikati Christelle Noelle, Gouado Inocent. (2014). Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon. International Journal of Nutrition and Food Sciences, 3(4), 251-258. https://doi.org/10.11648/j.ijnfs.20140304.14

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ACS Style

Maffo Tazoho Ghislain; Agbor Esther Etengeneng; Mekoudjou Nouwou Hermine Sonia; Kengne Sikati Christelle Noelle; Gouado Inocent. Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon. Int. J. Nutr. Food Sci. 2014, 3(4), 251-258. doi: 10.11648/j.ijnfs.20140304.14

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AMA Style

Maffo Tazoho Ghislain, Agbor Esther Etengeneng, Mekoudjou Nouwou Hermine Sonia, Kengne Sikati Christelle Noelle, Gouado Inocent. Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon. Int J Nutr Food Sci. 2014;3(4):251-258. doi: 10.11648/j.ijnfs.20140304.14

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@article{10.11648/j.ijnfs.20140304.14,
  author = {Maffo Tazoho Ghislain and Agbor Esther Etengeneng and Mekoudjou Nouwou Hermine Sonia and Kengne Sikati Christelle Noelle and Gouado Inocent},
  title = {Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {3},
  number = {4},
  pages = {251-258},
  doi = {10.11648/j.ijnfs.20140304.14},
  url = {https://doi.org/10.11648/j.ijnfs.20140304.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140304.14},
  abstract = {The chemical composition and protein quality of raw, boiled, toasted and soak-toasted roselle seeds harvested in the North (area I) and West (area II) regions of Cameroon were investigated in this study. To conduct the growth and nitrogen balance study, sixty albinos wistar rats of 21 to 25 days separated into ten groups of six rats/group were used and fed for fourteen days with 10% (w/w) isoproteinous experimental diets. These groups were Protein free diet (D0), egg white-standard reference protein diet (D1) and raw (D2I, D2II), boiled (D3I, D3II), toasted (D4I, D4II) and soak-toasted (D5II, D6II) roselle seed diets. Transaminases, serum creatinine, haematocrit, lipid parameters were also assessed in the rats. Results showed that crude protein, lipid, fiber and ash content of seed samples ranged respectively from 22 to 26, 18 to 22, 18 to 23 and 4.3 to 6.4%. The contents of Ca, Mg, K, Na, P, Fe and Zn were ranged from 1054 to 1920, 1670 to 2083, 26.45 to 272.7, 14 to 22, 10.58 to 90.78, 137.3 to 169.22 and 10.13 to 70.11 mg/kg respectively. Food intake was higher (P<0.05) for groups D1, D3I and D3II than for others groups. The rats fed with reference diet followed by boiled seed diets had the highest (P<0.05) weight gained, protein efficiency ratio (PER), net protein ratio (NPR), feed efficiency (FE), efficiency of food utilization (EFU) and true digestibility (TD). Aspartate aminotransferase and alanine aminotranferase were highest (p<0.05) in the rats fed raw roselle seeds. Compared to reference diet, the total cholesterol (TC), triglycerides (TG) and LDL-C of rats fed with diet D5II decreased while the HDL-C did not show any significant changes in these rats (p˃0.05). These findings show that roselle seeds cultivated in Cameroon have important amount of macronutrients and minerals. Proteins of roselle seeds are of good quality especially from the boiled seeds and are not influenced by the agro ecological region. Consumption of roselle seeds might be beneficial for cardio protective effect and no toxicity was observed as far as transaminases are concerned except that for the raw seeds which showed significant hepatotoxicity.},
 year = {2014}
}

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TY  - JOUR
T1  - Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon
AU  - Maffo Tazoho Ghislain
AU  - Agbor Esther Etengeneng
AU  - Mekoudjou Nouwou Hermine Sonia
AU  - Kengne Sikati Christelle Noelle
AU  - Gouado Inocent
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijnfs.20140304.14
DO  - 10.11648/j.ijnfs.20140304.14
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 251
EP  - 258
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20140304.14
AB  - The chemical composition and protein quality of raw, boiled, toasted and soak-toasted roselle seeds harvested in the North (area I) and West (area II) regions of Cameroon were investigated in this study. To conduct the growth and nitrogen balance study, sixty albinos wistar rats of 21 to 25 days separated into ten groups of six rats/group were used and fed for fourteen days with 10% (w/w) isoproteinous experimental diets. These groups were Protein free diet (D0), egg white-standard reference protein diet (D1) and raw (D2I, D2II), boiled (D3I, D3II), toasted (D4I, D4II) and soak-toasted (D5II, D6II) roselle seed diets. Transaminases, serum creatinine, haematocrit, lipid parameters were also assessed in the rats. Results showed that crude protein, lipid, fiber and ash content of seed samples ranged respectively from 22 to 26, 18 to 22, 18 to 23 and 4.3 to 6.4%. The contents of Ca, Mg, K, Na, P, Fe and Zn were ranged from 1054 to 1920, 1670 to 2083, 26.45 to 272.7, 14 to 22, 10.58 to 90.78, 137.3 to 169.22 and 10.13 to 70.11 mg/kg respectively. Food intake was higher (P<0.05) for groups D1, D3I and D3II than for others groups. The rats fed with reference diet followed by boiled seed diets had the highest (P<0.05) weight gained, protein efficiency ratio (PER), net protein ratio (NPR), feed efficiency (FE), efficiency of food utilization (EFU) and true digestibility (TD). Aspartate aminotransferase and alanine aminotranferase were highest (p<0.05) in the rats fed raw roselle seeds. Compared to reference diet, the total cholesterol (TC), triglycerides (TG) and LDL-C of rats fed with diet D5II decreased while the HDL-C did not show any significant changes in these rats (p˃0.05). These findings show that roselle seeds cultivated in Cameroon have important amount of macronutrients and minerals. Proteins of roselle seeds are of good quality especially from the boiled seeds and are not influenced by the agro ecological region. Consumption of roselle seeds might be beneficial for cardio protective effect and no toxicity was observed as far as transaminases are concerned except that for the raw seeds which showed significant hepatotoxicity.
VL  - 3
IS  - 4
ER  -

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Author Information

Maffo Tazoho Ghislain

Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Agbor Esther Etengeneng

Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Mekoudjou Nouwou Hermine Sonia

Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Kengne Sikati Christelle Noelle

Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon
Gouado Inocent

Department of Biochemistry, Faculty of science, University of Douala, Douala, Cameroon

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APA Style

Maffo Tazoho Ghislain, Agbor Esther Etengeneng, Mekoudjou Nouwou Hermine Sonia, Kengne Sikati Christelle Noelle, Gouado Inocent. (2014). Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon. International Journal of Nutrition and Food Sciences, 3(4), 251-258. https://doi.org/10.11648/j.ijnfs.20140304.14

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ACS Style

Maffo Tazoho Ghislain; Agbor Esther Etengeneng; Mekoudjou Nouwou Hermine Sonia; Kengne Sikati Christelle Noelle; Gouado Inocent. Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon. Int. J. Nutr. Food Sci. 2014, 3(4), 251-258. doi: 10.11648/j.ijnfs.20140304.14

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AMA Style

Maffo Tazoho Ghislain, Agbor Esther Etengeneng, Mekoudjou Nouwou Hermine Sonia, Kengne Sikati Christelle Noelle, Gouado Inocent. Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon. Int J Nutr Food Sci. 2014;3(4):251-258. doi: 10.11648/j.ijnfs.20140304.14

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@article{10.11648/j.ijnfs.20140304.14,
  author = {Maffo Tazoho Ghislain and Agbor Esther Etengeneng and Mekoudjou Nouwou Hermine Sonia and Kengne Sikati Christelle Noelle and Gouado Inocent},
  title = {Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon},
  journal = {International Journal of Nutrition and Food Sciences},
  volume = {3},
  number = {4},
  pages = {251-258},
  doi = {10.11648/j.ijnfs.20140304.14},
  url = {https://doi.org/10.11648/j.ijnfs.20140304.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20140304.14},
  abstract = {The chemical composition and protein quality of raw, boiled, toasted and soak-toasted roselle seeds harvested in the North (area I) and West (area II) regions of Cameroon were investigated in this study. To conduct the growth and nitrogen balance study, sixty albinos wistar rats of 21 to 25 days separated into ten groups of six rats/group were used and fed for fourteen days with 10% (w/w) isoproteinous experimental diets. These groups were Protein free diet (D0), egg white-standard reference protein diet (D1) and raw (D2I, D2II), boiled (D3I, D3II), toasted (D4I, D4II) and soak-toasted (D5II, D6II) roselle seed diets. Transaminases, serum creatinine, haematocrit, lipid parameters were also assessed in the rats. Results showed that crude protein, lipid, fiber and ash content of seed samples ranged respectively from 22 to 26, 18 to 22, 18 to 23 and 4.3 to 6.4%. The contents of Ca, Mg, K, Na, P, Fe and Zn were ranged from 1054 to 1920, 1670 to 2083, 26.45 to 272.7, 14 to 22, 10.58 to 90.78, 137.3 to 169.22 and 10.13 to 70.11 mg/kg respectively. Food intake was higher (P<0.05) for groups D1, D3I and D3II than for others groups. The rats fed with reference diet followed by boiled seed diets had the highest (P<0.05) weight gained, protein efficiency ratio (PER), net protein ratio (NPR), feed efficiency (FE), efficiency of food utilization (EFU) and true digestibility (TD). Aspartate aminotransferase and alanine aminotranferase were highest (p<0.05) in the rats fed raw roselle seeds. Compared to reference diet, the total cholesterol (TC), triglycerides (TG) and LDL-C of rats fed with diet D5II decreased while the HDL-C did not show any significant changes in these rats (p˃0.05). These findings show that roselle seeds cultivated in Cameroon have important amount of macronutrients and minerals. Proteins of roselle seeds are of good quality especially from the boiled seeds and are not influenced by the agro ecological region. Consumption of roselle seeds might be beneficial for cardio protective effect and no toxicity was observed as far as transaminases are concerned except that for the raw seeds which showed significant hepatotoxicity.},
 year = {2014}
}

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TY  - JOUR
T1  - Proximate and Mineral Composition, Protein Quality of Hibiscus Sabdariffa L. (Roselle) Seeds Cultivated in Two Agro Ecological Areas in Cameroon
AU  - Maffo Tazoho Ghislain
AU  - Agbor Esther Etengeneng
AU  - Mekoudjou Nouwou Hermine Sonia
AU  - Kengne Sikati Christelle Noelle
AU  - Gouado Inocent
Y1  - 2014/06/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ijnfs.20140304.14
DO  - 10.11648/j.ijnfs.20140304.14
T2  - International Journal of Nutrition and Food Sciences
JF  - International Journal of Nutrition and Food Sciences
JO  - International Journal of Nutrition and Food Sciences
SP  - 251
EP  - 258
PB  - Science Publishing Group
SN  - 2327-2716
UR  - https://doi.org/10.11648/j.ijnfs.20140304.14
AB  - The chemical composition and protein quality of raw, boiled, toasted and soak-toasted roselle seeds harvested in the North (area I) and West (area II) regions of Cameroon were investigated in this study. To conduct the growth and nitrogen balance study, sixty albinos wistar rats of 21 to 25 days separated into ten groups of six rats/group were used and fed for fourteen days with 10% (w/w) isoproteinous experimental diets. These groups were Protein free diet (D0), egg white-standard reference protein diet (D1) and raw (D2I, D2II), boiled (D3I, D3II), toasted (D4I, D4II) and soak-toasted (D5II, D6II) roselle seed diets. Transaminases, serum creatinine, haematocrit, lipid parameters were also assessed in the rats. Results showed that crude protein, lipid, fiber and ash content of seed samples ranged respectively from 22 to 26, 18 to 22, 18 to 23 and 4.3 to 6.4%. The contents of Ca, Mg, K, Na, P, Fe and Zn were ranged from 1054 to 1920, 1670 to 2083, 26.45 to 272.7, 14 to 22, 10.58 to 90.78, 137.3 to 169.22 and 10.13 to 70.11 mg/kg respectively. Food intake was higher (P<0.05) for groups D1, D3I and D3II than for others groups. The rats fed with reference diet followed by boiled seed diets had the highest (P<0.05) weight gained, protein efficiency ratio (PER), net protein ratio (NPR), feed efficiency (FE), efficiency of food utilization (EFU) and true digestibility (TD). Aspartate aminotransferase and alanine aminotranferase were highest (p<0.05) in the rats fed raw roselle seeds. Compared to reference diet, the total cholesterol (TC), triglycerides (TG) and LDL-C of rats fed with diet D5II decreased while the HDL-C did not show any significant changes in these rats (p˃0.05). These findings show that roselle seeds cultivated in Cameroon have important amount of macronutrients and minerals. Proteins of roselle seeds are of good quality especially from the boiled seeds and are not influenced by the agro ecological region. Consumption of roselle seeds might be beneficial for cardio protective effect and no toxicity was observed as far as transaminases are concerned except that for the raw seeds which showed significant hepatotoxicity.
VL  - 3
IS  - 4
ER  -

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