The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise

Ehiaghe Friday Alfred; Onyenekwe Charles Chinedum; Ifeanyichukwu Martins; Akosile Christopher Olusanjo; Ehiaghe Imuetiyan Joy; Nancy Ibeh Chitogu; Ukibe Nkiruka Rose; Osakue Nosa; Ekpe Elijah Chukwuemeka

doi:doi:10.11648/j.iji.20170504.13

The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise

Ehiaghe Friday Alfred, Onyenekwe Charles Chinedum, Ifeanyichukwu Martins, Akosile Christopher Olusanjo, Ehiaghe Imuetiyan Joy, Nancy Ibeh Chitogu, Ukibe Nkiruka Rose, Osakue Nosa, Ekpe Elijah Chukwuemeka

Published in International Journal of Immunology (Volume 5, Issue 4)

Received: 3 July 2017 Accepted: 18 July 2017 Published: 11 August 2017

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Abstract

This study was designed to determine the expression patterns of some pro-inflammatory and anti-inflammatory cytokine genes in some young male undergraduates that passed through exhaustive exercise using the Bruce protocol for sub-maximal acute exercise as studies have shown that exhaustive exercise activates the release of multiple cytokine genes which modulate the immune responses of stressed individuals. Twenty five (25) healthy young male undergraduates with an average age of 24.3±3 years and body mass index of 22.7±1.8 (Kg/m²) participated fully in the study. Blood samples were collected from the participants before commencement of the study, at 1 hour, 4 hours and 24 hours post exercise. Tumor necrosis factor α, interferon gamma and interleukin-10 genes expression patterns were detected using reverse transcriptase polymerase chain reaction methods. Serum cortisol (nmol/L) using enzyme-linked immunosorbent assay method. The study revealed that eleven (11) out of the twenty-five (25) participants had their tumor necrosis factor α genes up-regulated at 1 hour post exercise and sustained till 24 hours post exercise. Moreover, interferon gamma and interleukin-10 genes were up-regulated at 4 hours post exercise and sustained till 24 hours post exercise in most of the subjects. This study also observed that the cortisol level of the exercised subjects was significantly higher at 1 hour post acute exercise when compared with pre-exercise stage. Acute exercise was associated with an enhanced lymphocytic cell functions which are evidence in the up-regulation patterns of both lymphocytic pro-inflammatory cytokine and anti-inflammatory cytokine genes post exercise.

Published in	International Journal of Immunology (Volume 5, Issue 4)
DOI	10.11648/j.iji.20170504.13
Page(s)	74-79
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Tumor Necrosis Factor Alpha Genes, Exercise, Lymphocytic Cell Functions, Cytokines, Cortisol

References

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APA Style

Ehiaghe Friday Alfred, Onyenekwe Charles Chinedum, Ifeanyichukwu Martins, Akosile Christopher Olusanjo, Ehiaghe Imuetiyan Joy, et al. (2017). The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise. International Journal of Immunology, 5(4), 74-79. https://doi.org/10.11648/j.iji.20170504.13

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ACS Style

Ehiaghe Friday Alfred; Onyenekwe Charles Chinedum; Ifeanyichukwu Martins; Akosile Christopher Olusanjo; Ehiaghe Imuetiyan Joy, et al. The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise. Int. J. Immunol. 2017, 5(4), 74-79. doi: 10.11648/j.iji.20170504.13

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AMA Style

Ehiaghe Friday Alfred, Onyenekwe Charles Chinedum, Ifeanyichukwu Martins, Akosile Christopher Olusanjo, Ehiaghe Imuetiyan Joy, et al. The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise. Int J Immunol. 2017;5(4):74-79. doi: 10.11648/j.iji.20170504.13

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@article{10.11648/j.iji.20170504.13,
  author = {Ehiaghe Friday Alfred and Onyenekwe Charles Chinedum and Ifeanyichukwu Martins and Akosile Christopher Olusanjo and Ehiaghe Imuetiyan Joy and Nancy Ibeh Chitogu and Ukibe Nkiruka Rose and Osakue Nosa and Ekpe Elijah Chukwuemeka},
  title = {The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise},
  journal = {International Journal of Immunology},
  volume = {5},
  number = {4},
  pages = {74-79},
  doi = {10.11648/j.iji.20170504.13},
  url = {https://doi.org/10.11648/j.iji.20170504.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20170504.13},
  abstract = {This study was designed to determine the expression patterns of some pro-inflammatory and anti-inflammatory cytokine genes in some young male undergraduates that passed through exhaustive exercise using the Bruce protocol for sub-maximal acute exercise as studies have shown that exhaustive exercise activates the release of multiple cytokine genes which modulate the immune responses of stressed individuals. Twenty five (25) healthy young male undergraduates with an average age of 24.3±3 years and body mass index of 22.7±1.8 (Kg/m2) participated fully in the study. Blood samples were collected from the participants before commencement of the study, at 1 hour, 4 hours and 24 hours post exercise. Tumor necrosis factor α, interferon gamma and interleukin-10 genes expression patterns were detected using reverse transcriptase polymerase chain reaction methods. Serum cortisol (nmol/L) using enzyme-linked immunosorbent assay method. The study revealed that eleven (11) out of the twenty-five (25) participants had their tumor necrosis factor α genes up-regulated at 1 hour post exercise and sustained till 24 hours post exercise. Moreover, interferon gamma and interleukin-10 genes were up-regulated at 4 hours post exercise and sustained till 24 hours post exercise in most of the subjects. This study also observed that the cortisol level of the exercised subjects was significantly higher at 1 hour post acute exercise when compared with pre-exercise stage. Acute exercise was associated with an enhanced lymphocytic cell functions which are evidence in the up-regulation patterns of both lymphocytic pro-inflammatory cytokine and anti-inflammatory cytokine genes post exercise.},
 year = {2017}
}

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TY  - JOUR
T1  - The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise
AU  - Ehiaghe Friday Alfred
AU  - Onyenekwe Charles Chinedum
AU  - Ifeanyichukwu Martins
AU  - Akosile Christopher Olusanjo
AU  - Ehiaghe Imuetiyan Joy
AU  - Nancy Ibeh Chitogu
AU  - Ukibe Nkiruka Rose
AU  - Osakue Nosa
AU  - Ekpe Elijah Chukwuemeka
Y1  - 2017/08/11
PY  - 2017
N1  - https://doi.org/10.11648/j.iji.20170504.13
DO  - 10.11648/j.iji.20170504.13
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 74
EP  - 79
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20170504.13
AB  - This study was designed to determine the expression patterns of some pro-inflammatory and anti-inflammatory cytokine genes in some young male undergraduates that passed through exhaustive exercise using the Bruce protocol for sub-maximal acute exercise as studies have shown that exhaustive exercise activates the release of multiple cytokine genes which modulate the immune responses of stressed individuals. Twenty five (25) healthy young male undergraduates with an average age of 24.3±3 years and body mass index of 22.7±1.8 (Kg/m2) participated fully in the study. Blood samples were collected from the participants before commencement of the study, at 1 hour, 4 hours and 24 hours post exercise. Tumor necrosis factor α, interferon gamma and interleukin-10 genes expression patterns were detected using reverse transcriptase polymerase chain reaction methods. Serum cortisol (nmol/L) using enzyme-linked immunosorbent assay method. The study revealed that eleven (11) out of the twenty-five (25) participants had their tumor necrosis factor α genes up-regulated at 1 hour post exercise and sustained till 24 hours post exercise. Moreover, interferon gamma and interleukin-10 genes were up-regulated at 4 hours post exercise and sustained till 24 hours post exercise in most of the subjects. This study also observed that the cortisol level of the exercised subjects was significantly higher at 1 hour post acute exercise when compared with pre-exercise stage. Acute exercise was associated with an enhanced lymphocytic cell functions which are evidence in the up-regulation patterns of both lymphocytic pro-inflammatory cytokine and anti-inflammatory cytokine genes post exercise.
VL  - 5
IS  - 4
ER  -

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Author Information

Ehiaghe Friday Alfred

Department of Hematology, College of Health Sciences, Igbinedion University, Okada, Nigeria
Onyenekwe Charles Chinedum

Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Ifeanyichukwu Martins

Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Akosile Christopher Olusanjo

Department of Medical Rehabilitation, Nnamdi Azikiwe University, Awka, Nigeria
Ehiaghe Imuetiyan Joy

Lahor Research and Medical Centre, Benin City, Nigeria
Nancy Ibeh Chitogu

Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Ukibe Nkiruka Rose

Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Osakue Nosa

Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Ekpe Elijah Chukwuemeka

Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria

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Plain Text BibTeX RIS

APA Style

Ehiaghe Friday Alfred, Onyenekwe Charles Chinedum, Ifeanyichukwu Martins, Akosile Christopher Olusanjo, Ehiaghe Imuetiyan Joy, et al. (2017). The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise. International Journal of Immunology, 5(4), 74-79. https://doi.org/10.11648/j.iji.20170504.13

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ACS Style

Ehiaghe Friday Alfred; Onyenekwe Charles Chinedum; Ifeanyichukwu Martins; Akosile Christopher Olusanjo; Ehiaghe Imuetiyan Joy, et al. The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise. Int. J. Immunol. 2017, 5(4), 74-79. doi: 10.11648/j.iji.20170504.13

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AMA Style

Ehiaghe Friday Alfred, Onyenekwe Charles Chinedum, Ifeanyichukwu Martins, Akosile Christopher Olusanjo, Ehiaghe Imuetiyan Joy, et al. The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise. Int J Immunol. 2017;5(4):74-79. doi: 10.11648/j.iji.20170504.13

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@article{10.11648/j.iji.20170504.13,
  author = {Ehiaghe Friday Alfred and Onyenekwe Charles Chinedum and Ifeanyichukwu Martins and Akosile Christopher Olusanjo and Ehiaghe Imuetiyan Joy and Nancy Ibeh Chitogu and Ukibe Nkiruka Rose and Osakue Nosa and Ekpe Elijah Chukwuemeka},
  title = {The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise},
  journal = {International Journal of Immunology},
  volume = {5},
  number = {4},
  pages = {74-79},
  doi = {10.11648/j.iji.20170504.13},
  url = {https://doi.org/10.11648/j.iji.20170504.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20170504.13},
  abstract = {This study was designed to determine the expression patterns of some pro-inflammatory and anti-inflammatory cytokine genes in some young male undergraduates that passed through exhaustive exercise using the Bruce protocol for sub-maximal acute exercise as studies have shown that exhaustive exercise activates the release of multiple cytokine genes which modulate the immune responses of stressed individuals. Twenty five (25) healthy young male undergraduates with an average age of 24.3±3 years and body mass index of 22.7±1.8 (Kg/m2) participated fully in the study. Blood samples were collected from the participants before commencement of the study, at 1 hour, 4 hours and 24 hours post exercise. Tumor necrosis factor α, interferon gamma and interleukin-10 genes expression patterns were detected using reverse transcriptase polymerase chain reaction methods. Serum cortisol (nmol/L) using enzyme-linked immunosorbent assay method. The study revealed that eleven (11) out of the twenty-five (25) participants had their tumor necrosis factor α genes up-regulated at 1 hour post exercise and sustained till 24 hours post exercise. Moreover, interferon gamma and interleukin-10 genes were up-regulated at 4 hours post exercise and sustained till 24 hours post exercise in most of the subjects. This study also observed that the cortisol level of the exercised subjects was significantly higher at 1 hour post acute exercise when compared with pre-exercise stage. Acute exercise was associated with an enhanced lymphocytic cell functions which are evidence in the up-regulation patterns of both lymphocytic pro-inflammatory cytokine and anti-inflammatory cytokine genes post exercise.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - The Expression Patterns of Some Pro-inflammatory and Anti-inflammatory Cytokines Genes Pre and Post Acute Exercise
AU  - Ehiaghe Friday Alfred
AU  - Onyenekwe Charles Chinedum
AU  - Ifeanyichukwu Martins
AU  - Akosile Christopher Olusanjo
AU  - Ehiaghe Imuetiyan Joy
AU  - Nancy Ibeh Chitogu
AU  - Ukibe Nkiruka Rose
AU  - Osakue Nosa
AU  - Ekpe Elijah Chukwuemeka
Y1  - 2017/08/11
PY  - 2017
N1  - https://doi.org/10.11648/j.iji.20170504.13
DO  - 10.11648/j.iji.20170504.13
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 74
EP  - 79
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20170504.13
AB  - This study was designed to determine the expression patterns of some pro-inflammatory and anti-inflammatory cytokine genes in some young male undergraduates that passed through exhaustive exercise using the Bruce protocol for sub-maximal acute exercise as studies have shown that exhaustive exercise activates the release of multiple cytokine genes which modulate the immune responses of stressed individuals. Twenty five (25) healthy young male undergraduates with an average age of 24.3±3 years and body mass index of 22.7±1.8 (Kg/m2) participated fully in the study. Blood samples were collected from the participants before commencement of the study, at 1 hour, 4 hours and 24 hours post exercise. Tumor necrosis factor α, interferon gamma and interleukin-10 genes expression patterns were detected using reverse transcriptase polymerase chain reaction methods. Serum cortisol (nmol/L) using enzyme-linked immunosorbent assay method. The study revealed that eleven (11) out of the twenty-five (25) participants had their tumor necrosis factor α genes up-regulated at 1 hour post exercise and sustained till 24 hours post exercise. Moreover, interferon gamma and interleukin-10 genes were up-regulated at 4 hours post exercise and sustained till 24 hours post exercise in most of the subjects. This study also observed that the cortisol level of the exercised subjects was significantly higher at 1 hour post acute exercise when compared with pre-exercise stage. Acute exercise was associated with an enhanced lymphocytic cell functions which are evidence in the up-regulation patterns of both lymphocytic pro-inflammatory cytokine and anti-inflammatory cytokine genes post exercise.
VL  - 5
IS  - 4
ER  -

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