The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development

Christina Rubino; Anika Chowdhury; Cassandra Greco; Benjamin Honigsfeld; Barbara Pepe; Noelle Cutter

doi:doi:10.11648/j.ijbse.20200804.12

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The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development

Christina Rubino, Anika Chowdhury, Cassandra Greco, Benjamin Honigsfeld, Barbara Pepe, Noelle Cutter

Published in International Journal of Biomedical Science and Engineering (Volume 8, Issue 4)

Received: 11 November 2020 Accepted: 2 December 2020 Published: 11 December 2020

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Abstract

Establish the role epigenetic methylation plays in the segregation of Medulloblastoma subgroups, through pathway analysis in the literature and cancer genome Atlas publicly available data. The goal is to develop an in-vitro assay to study therapeutic responses for subgroups 3 and 4. Functional analysis assays will be used to determine a mechanism for chemoresistance in specific subgroups. In the current study, we assessed Medulloblastoma group 3 (D341) and Group 4 (D283) cell lines using the DNA isolation kit and angiogenesis kits which allows for amplification via PCR and visualize tube formation for tumor nutrients. Twist 1 fended were measured and recorded with the methylation Gold. Group 3 MB cell lines treated with chemotherapy reagents demonstrate altered methylation profiles, angiogenic tube formation increase, a decrease in apoptosis, and underwent phenotypic changes to overcome resistance. Gel electrophoresis results indicated consistent Twist-1 expression in the DAOY+cisplatin cell line in comparison to the control. Cells treated with chemotherapeutic agents evade the mechanisms of apoptosis resulting in angiogenesis. We identified a possible correlation between cisplatin treatment, alteration of methylation patterns, and an increase of Twist-1 gene expression. Twist-1 previously identified to be involved in angiogenesis and cisplatin resistance (Roberts et al. 2016), this supports our hypothesis that alterations in methylation patterns result in an increase in overall chemoresistance.

Published in	International Journal of Biomedical Science and Engineering (Volume 8, Issue 4)
DOI	10.11648/j.ijbse.20200804.12
Page(s)	51-57
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Methylation, Angiogenesis, Chemo Resistant, Apoptosis

References

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APA Style

Christina Rubino, Anika Chowdhury, Cassandra Greco, Benjamin Honigsfeld, Barbara Pepe, et al. (2020). The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development. International Journal of Biomedical Science and Engineering, 8(4), 51-57. https://doi.org/10.11648/j.ijbse.20200804.12

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ACS Style

Christina Rubino; Anika Chowdhury; Cassandra Greco; Benjamin Honigsfeld; Barbara Pepe, et al. The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development. Int. J. Biomed. Sci. Eng. 2020, 8(4), 51-57. doi: 10.11648/j.ijbse.20200804.12

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AMA Style

Christina Rubino, Anika Chowdhury, Cassandra Greco, Benjamin Honigsfeld, Barbara Pepe, et al. The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development. Int J Biomed Sci Eng. 2020;8(4):51-57. doi: 10.11648/j.ijbse.20200804.12

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@article{10.11648/j.ijbse.20200804.12,
  author = {Christina Rubino and Anika Chowdhury and Cassandra Greco and Benjamin Honigsfeld and Barbara Pepe and Noelle Cutter},
  title = {The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {8},
  number = {4},
  pages = {51-57},
  doi = {10.11648/j.ijbse.20200804.12},
  url = {https://doi.org/10.11648/j.ijbse.20200804.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20200804.12},
  abstract = {Establish the role epigenetic methylation plays in the segregation of Medulloblastoma subgroups, through pathway analysis in the literature and cancer genome Atlas publicly available data. The goal is to develop an in-vitro assay to study therapeutic responses for subgroups 3 and 4. Functional analysis assays will be used to determine a mechanism for chemoresistance in specific subgroups. In the current study, we assessed Medulloblastoma group 3 (D341) and Group 4 (D283) cell lines using the DNA isolation kit and angiogenesis kits which allows for amplification via PCR and visualize tube formation for tumor nutrients. Twist 1 fended were measured and recorded with the methylation Gold. Group 3 MB cell lines treated with chemotherapy reagents demonstrate altered methylation profiles, angiogenic tube formation increase, a decrease in apoptosis, and underwent phenotypic changes to overcome resistance. Gel electrophoresis results indicated consistent Twist-1 expression in the DAOY+cisplatin cell line in comparison to the control. Cells treated with chemotherapeutic agents evade the mechanisms of apoptosis resulting in angiogenesis. We identified a possible correlation between cisplatin treatment, alteration of methylation patterns, and an increase of Twist-1 gene expression. Twist-1 previously identified to be involved in angiogenesis and cisplatin resistance (Roberts et al. 2016), this supports our hypothesis that alterations in methylation patterns result in an increase in overall chemoresistance.},
 year = {2020}
}

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TY  - JOUR
T1  - The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development
AU  - Christina Rubino
AU  - Anika Chowdhury
AU  - Cassandra Greco
AU  - Benjamin Honigsfeld
AU  - Barbara Pepe
AU  - Noelle Cutter
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DO  - 10.11648/j.ijbse.20200804.12
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
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PB  - Science Publishing Group
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UR  - https://doi.org/10.11648/j.ijbse.20200804.12
AB  - Establish the role epigenetic methylation plays in the segregation of Medulloblastoma subgroups, through pathway analysis in the literature and cancer genome Atlas publicly available data. The goal is to develop an in-vitro assay to study therapeutic responses for subgroups 3 and 4. Functional analysis assays will be used to determine a mechanism for chemoresistance in specific subgroups. In the current study, we assessed Medulloblastoma group 3 (D341) and Group 4 (D283) cell lines using the DNA isolation kit and angiogenesis kits which allows for amplification via PCR and visualize tube formation for tumor nutrients. Twist 1 fended were measured and recorded with the methylation Gold. Group 3 MB cell lines treated with chemotherapy reagents demonstrate altered methylation profiles, angiogenic tube formation increase, a decrease in apoptosis, and underwent phenotypic changes to overcome resistance. Gel electrophoresis results indicated consistent Twist-1 expression in the DAOY+cisplatin cell line in comparison to the control. Cells treated with chemotherapeutic agents evade the mechanisms of apoptosis resulting in angiogenesis. We identified a possible correlation between cisplatin treatment, alteration of methylation patterns, and an increase of Twist-1 gene expression. Twist-1 previously identified to be involved in angiogenesis and cisplatin resistance (Roberts et al. 2016), this supports our hypothesis that alterations in methylation patterns result in an increase in overall chemoresistance.
VL  - 8
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Author Information

Christina Rubino

Department of Biological Sciences, Molloy College, Rockville Centre, New York, United States
Anika Chowdhury

Department of Biological Sciences, Molloy College, Rockville Centre, New York, United States
Cassandra Greco

Department of Biological Sciences, Molloy College, Rockville Centre, New York, United States
Benjamin Honigsfeld

Department of Biological Sciences, Molloy College, Rockville Centre, New York, United States
Barbara Pepe

Department of Biological Sciences, Molloy College, Rockville Centre, New York, United States
Noelle Cutter

Department of Biological Sciences, Molloy College, Rockville Centre, New York, United States

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APA Style

Christina Rubino, Anika Chowdhury, Cassandra Greco, Benjamin Honigsfeld, Barbara Pepe, et al. (2020). The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development. International Journal of Biomedical Science and Engineering, 8(4), 51-57. https://doi.org/10.11648/j.ijbse.20200804.12

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ACS Style

Christina Rubino; Anika Chowdhury; Cassandra Greco; Benjamin Honigsfeld; Barbara Pepe, et al. The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development. Int. J. Biomed. Sci. Eng. 2020, 8(4), 51-57. doi: 10.11648/j.ijbse.20200804.12

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AMA Style

Christina Rubino, Anika Chowdhury, Cassandra Greco, Benjamin Honigsfeld, Barbara Pepe, et al. The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development. Int J Biomed Sci Eng. 2020;8(4):51-57. doi: 10.11648/j.ijbse.20200804.12

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@article{10.11648/j.ijbse.20200804.12,
  author = {Christina Rubino and Anika Chowdhury and Cassandra Greco and Benjamin Honigsfeld and Barbara Pepe and Noelle Cutter},
  title = {The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development},
  journal = {International Journal of Biomedical Science and Engineering},
  volume = {8},
  number = {4},
  pages = {51-57},
  doi = {10.11648/j.ijbse.20200804.12},
  url = {https://doi.org/10.11648/j.ijbse.20200804.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbse.20200804.12},
  abstract = {Establish the role epigenetic methylation plays in the segregation of Medulloblastoma subgroups, through pathway analysis in the literature and cancer genome Atlas publicly available data. The goal is to develop an in-vitro assay to study therapeutic responses for subgroups 3 and 4. Functional analysis assays will be used to determine a mechanism for chemoresistance in specific subgroups. In the current study, we assessed Medulloblastoma group 3 (D341) and Group 4 (D283) cell lines using the DNA isolation kit and angiogenesis kits which allows for amplification via PCR and visualize tube formation for tumor nutrients. Twist 1 fended were measured and recorded with the methylation Gold. Group 3 MB cell lines treated with chemotherapy reagents demonstrate altered methylation profiles, angiogenic tube formation increase, a decrease in apoptosis, and underwent phenotypic changes to overcome resistance. Gel electrophoresis results indicated consistent Twist-1 expression in the DAOY+cisplatin cell line in comparison to the control. Cells treated with chemotherapeutic agents evade the mechanisms of apoptosis resulting in angiogenesis. We identified a possible correlation between cisplatin treatment, alteration of methylation patterns, and an increase of Twist-1 gene expression. Twist-1 previously identified to be involved in angiogenesis and cisplatin resistance (Roberts et al. 2016), this supports our hypothesis that alterations in methylation patterns result in an increase in overall chemoresistance.},
 year = {2020}
}

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TY  - JOUR
T1  - The Effects of DNA Methylation on Medulloblastoma Cell Survivability and Development
AU  - Christina Rubino
AU  - Anika Chowdhury
AU  - Cassandra Greco
AU  - Benjamin Honigsfeld
AU  - Barbara Pepe
AU  - Noelle Cutter
Y1  - 2020/12/11
PY  - 2020
N1  - https://doi.org/10.11648/j.ijbse.20200804.12
DO  - 10.11648/j.ijbse.20200804.12
T2  - International Journal of Biomedical Science and Engineering
JF  - International Journal of Biomedical Science and Engineering
JO  - International Journal of Biomedical Science and Engineering
SP  - 51
EP  - 57
PB  - Science Publishing Group
SN  - 2376-7235
UR  - https://doi.org/10.11648/j.ijbse.20200804.12
AB  - Establish the role epigenetic methylation plays in the segregation of Medulloblastoma subgroups, through pathway analysis in the literature and cancer genome Atlas publicly available data. The goal is to develop an in-vitro assay to study therapeutic responses for subgroups 3 and 4. Functional analysis assays will be used to determine a mechanism for chemoresistance in specific subgroups. In the current study, we assessed Medulloblastoma group 3 (D341) and Group 4 (D283) cell lines using the DNA isolation kit and angiogenesis kits which allows for amplification via PCR and visualize tube formation for tumor nutrients. Twist 1 fended were measured and recorded with the methylation Gold. Group 3 MB cell lines treated with chemotherapy reagents demonstrate altered methylation profiles, angiogenic tube formation increase, a decrease in apoptosis, and underwent phenotypic changes to overcome resistance. Gel electrophoresis results indicated consistent Twist-1 expression in the DAOY+cisplatin cell line in comparison to the control. Cells treated with chemotherapeutic agents evade the mechanisms of apoptosis resulting in angiogenesis. We identified a possible correlation between cisplatin treatment, alteration of methylation patterns, and an increase of Twist-1 gene expression. Twist-1 previously identified to be involved in angiogenesis and cisplatin resistance (Roberts et al. 2016), this supports our hypothesis that alterations in methylation patterns result in an increase in overall chemoresistance.
VL  - 8
IS  - 4
ER  -

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