In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents

Nasir Hassan Wagini; Mohamed Said Abbas; Amira Shawky Soliman; Yasser Adel Hanafy; El-Saady Mohamed Badawy

doi:doi:10.11648/j.ajcem.20140203.13

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In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents

Nasir Hassan Wagini, Mohamed Said Abbas, Amira Shawky Soliman, Yasser Adel Hanafy, El-Saady Mohamed Badawy

Published in American Journal of Clinical and Experimental Medicine (Volume 2, Issue 3)

Received: 4 May 2014 Accepted: 17 May 2014 Published: 30 May 2014

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Abstract

The study was carried out to identify the etiological agents causing ringworm, evaluate the in vitro and in vivo antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the identified etiological agents, with a minimum inhibitory concentration of 625µg/ml except Microsporum canis which resisted the minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected goats. First, second and third group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated and negative control.

Published in	American Journal of Clinical and Experimental Medicine (Volume 2, Issue 3)
DOI	10.11648/j.ajcem.20140203.13
Page(s)	51-58
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Lawsonia Inermis, Antifungal, Trichophyton, Microsporum, Clotrimazole

References

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[3]	Shahitha S, Sa-ranya M and Poornima K. Isolation and Identification of Dermatophytes from Clinical Samples and Antidermatophytic Activity of Lawsonia inermis (Henna plant) International Journal of Pharma-ceutical and Chemical Sciences 2013; 2(2):1050.
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[16]	Mansour-Djaalab H, Kahlouche-Riachi F, Djerrou Z, Serakta-Delmi, M, Hamimed, S, et al. In vitro evaluation of antifungal effects of Lawsonia inermis, Pistacia lentiscus and Juglans regia. International Journal of Medicinal and Aromatic Plants 2012; 2(2): 263-268.
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APA Style

Nasir Hassan Wagini, Mohamed Said Abbas, Amira Shawky Soliman, Yasser Adel Hanafy, El-Saady Mohamed Badawy. (2014). In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. American Journal of Clinical and Experimental Medicine, 2(3), 51-58. https://doi.org/10.11648/j.ajcem.20140203.13

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ACS Style

Nasir Hassan Wagini; Mohamed Said Abbas; Amira Shawky Soliman; Yasser Adel Hanafy; El-Saady Mohamed Badawy. In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. Am. J. Clin. Exp. Med. 2014, 2(3), 51-58. doi: 10.11648/j.ajcem.20140203.13

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AMA Style

Nasir Hassan Wagini, Mohamed Said Abbas, Amira Shawky Soliman, Yasser Adel Hanafy, El-Saady Mohamed Badawy. In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. Am J Clin Exp Med. 2014;2(3):51-58. doi: 10.11648/j.ajcem.20140203.13

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@article{10.11648/j.ajcem.20140203.13,
  author = {Nasir Hassan Wagini and Mohamed Said Abbas and Amira Shawky Soliman and Yasser Adel Hanafy and El-Saady Mohamed Badawy},
  title = {In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {3},
  pages = {51-58},
  doi = {10.11648/j.ajcem.20140203.13},
  url = {https://doi.org/10.11648/j.ajcem.20140203.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140203.13},
  abstract = {The study was carried out to identify the etiological agents causing ringworm, evaluate the in vitro and in vivo antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the identified etiological agents, with a minimum inhibitory concentration of 625µg/ml except Microsporum canis which resisted the minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected goats. First, second and third  group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated and negative control.},
 year = {2014}
}

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TY  - JOUR
T1  - In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents
AU  - Nasir Hassan Wagini
AU  - Mohamed Said Abbas
AU  - Amira Shawky Soliman
AU  - Yasser Adel Hanafy
AU  - El-Saady Mohamed Badawy
Y1  - 2014/05/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajcem.20140203.13
DO  - 10.11648/j.ajcem.20140203.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 51
EP  - 58
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20140203.13
AB  - The study was carried out to identify the etiological agents causing ringworm, evaluate the in vitro and in vivo antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the identified etiological agents, with a minimum inhibitory concentration of 625µg/ml except Microsporum canis which resisted the minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected goats. First, second and third  group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated and negative control.
VL  - 2
IS  - 3
ER  -

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Author Information

Nasir Hassan Wagini

Department of Biology, Faculty of Natural and Applied Sciences, Umaru Musa Yar’adua University, Katsina, Nigeria
Mohamed Said Abbas

Department of Natural Resources, Institute of African Research and Studies, Cairo University, Cairo, Egypt
Amira Shawky Soliman

Department of Natural Resources, Institute of African Research and Studies, Cairo University, Cairo, Egypt
Yasser Adel Hanafy

Medical and Aromatic Plants Unit, Desert Research Center, Cairo, Egypt
El-Saady Mohamed Badawy

Medicinal and Ornamental Plant, Faculty of Agriculture, Cairo University, Cairo, Egypt

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APA Style

Nasir Hassan Wagini, Mohamed Said Abbas, Amira Shawky Soliman, Yasser Adel Hanafy, El-Saady Mohamed Badawy. (2014). In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. American Journal of Clinical and Experimental Medicine, 2(3), 51-58. https://doi.org/10.11648/j.ajcem.20140203.13

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ACS Style

Nasir Hassan Wagini; Mohamed Said Abbas; Amira Shawky Soliman; Yasser Adel Hanafy; El-Saady Mohamed Badawy. In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. Am. J. Clin. Exp. Med. 2014, 2(3), 51-58. doi: 10.11648/j.ajcem.20140203.13

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AMA Style

Nasir Hassan Wagini, Mohamed Said Abbas, Amira Shawky Soliman, Yasser Adel Hanafy, El-Saady Mohamed Badawy. In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents. Am J Clin Exp Med. 2014;2(3):51-58. doi: 10.11648/j.ajcem.20140203.13

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@article{10.11648/j.ajcem.20140203.13,
  author = {Nasir Hassan Wagini and Mohamed Said Abbas and Amira Shawky Soliman and Yasser Adel Hanafy and El-Saady Mohamed Badawy},
  title = {In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {2},
  number = {3},
  pages = {51-58},
  doi = {10.11648/j.ajcem.20140203.13},
  url = {https://doi.org/10.11648/j.ajcem.20140203.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20140203.13},
  abstract = {The study was carried out to identify the etiological agents causing ringworm, evaluate the in vitro and in vivo antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the identified etiological agents, with a minimum inhibitory concentration of 625µg/ml except Microsporum canis which resisted the minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected goats. First, second and third  group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated and negative control.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - In Vitro and in Vivo Anti Dermatophytes Activity of Lawsonia Inermis L. (Henna) Leaves against Ringworm and Its Etiological Agents
AU  - Nasir Hassan Wagini
AU  - Mohamed Said Abbas
AU  - Amira Shawky Soliman
AU  - Yasser Adel Hanafy
AU  - El-Saady Mohamed Badawy
Y1  - 2014/05/30
PY  - 2014
N1  - https://doi.org/10.11648/j.ajcem.20140203.13
DO  - 10.11648/j.ajcem.20140203.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 51
EP  - 58
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20140203.13
AB  - The study was carried out to identify the etiological agents causing ringworm, evaluate the in vitro and in vivo antifungal activity of Lawsonia inermis L. In vitro study was carried out using agar dilution method. In a total of 50 clinical samples, 4 different species were identified namely; Microsporum canis, Trichophyton tonsurans, Trichophyton rubrum, and Trichophyton mentagrophytes. However, the results showed that hexane extract exerted a strong antifungal activity against all the identified etiological agents, with a minimum inhibitory concentration of 625µg/ml except Microsporum canis which resisted the minimum concentration but susceptible to the higher concentrations. The in vivo study was carried out using 15 naturally infected goats. First, second and third  group of animals were treated with henna paste, aqueous and ethanolic extract respectively, fourth group were treated with clotrimazole as a positive control while negative control (fifth) group were left untreated with neither henna nor clotrimazole. The treatments were compared and the results showed that henna paste had the highest efficacy against all the types of ringworm tested compared to the remaining treatments. Disappearance of lesion and complete repair of the hair was observed at 30 days after treatment. Significantly similar result was observed in the group of animals treated with aqueous extract, ethanol extract, and clotrimazole in all the parameters. Significant different was only observed between groups treated and negative control.
VL  - 2
IS  - 3
ER  -

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