Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and <i>Toxoplasma gondii</i> IgG Antibodies at Prenatal Screening

Genco Francesca; Meroni Valeria; De Silvestri Annalisa; Bouthry Elise

doi:doi:10.11648/j.ajbio.20200801.13

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Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening

Genco Francesca, Meroni Valeria, De Silvestri Annalisa, Bouthry Elise

Published in American Journal of BioScience (Volume 8, Issue 1)

Received: 6 March 2020 Accepted: 31 March 2020 Published: 13 April 2020

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Abstract

Toxoplasma gondii (T. gondii) and cytomegalovirus (CMV) infections are typically asymptomatic infections, but they can have serious consequences mainly in newborns and immunocompromised patients. In many parts of the world, these infections are routinely screened during pregnancy (toxoplasmosis) and, in others, high-risk individuals are tested using fully automated screening assays. In this study, we investigated the performance of the three fully automated immunoassays, LIAISON^® XL DiaSorin, Abbott Architect and Roche Cobas^®, for the determination of specific IgG antibodies to Cytomegalovirus and Toxoplasma gondii in human serum or plasma samples in terms of prevalence of CMV and Toxo IgG detected, and both sensitivity and specificity. Performance of the LIAISON^® assays was investigated compared to two other assays, ARCHITECT (CMV IgG and Toxo IgG assays) and Cobas^® (CMV IgG and Toxo IgG assays). Discrepant anti CMV IgG and anti Toxoplasma IgG samples were tested for IgM to CMV and Toxoplasma to exclude early acute infection where IgG could be detected differently by the methods. Overall, for both CMV IgG and Toxo IgG, the LIAISON^® assay was better than both the Cobas^® and ARCHITECT assays in terms of CMV and Toxo IgG detected, and both diagnostic sensitivity and specificity performance although the difference is statistically significant only compared to Cobas^®.

Published in	American Journal of BioScience (Volume 8, Issue 1)
DOI	10.11648/j.ajbio.20200801.13
Page(s)	15-19
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Toxoplasma gondii, Cytomegalovirus, Prenatal Screening, Anti-cytomegalovirus IgG Antibodies, Anti-toxoplasma IgG Antibodies

References

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[2]	Kenneson A and Cannon MJ. Review and meta-analysis of the epidemiology of congenital cytomegalovirus (CMV) infection. Rev Med Virol 2007; 17 (4): 253-276.
[3]	Lewis JM, Clifford S, et al. Toxoplasmosis in immunosuppressed patients. Rheumatology (Oxford) 2015; 54 (11): 1939-1940.
[4]	Sissons JG and Wills MR. How understanding immunology contributes to managing CMV disease in immunosuppressed patients: now and in future. Med Microbiol Immunol 2015; 204 (3): 307-316.
[5]	Neu N, Duchon J, et al. TORCH infections. Clin Perinatol 2015; 42 (1): 77-103, viii.
[6]	McAuley JB. Congenital Toxoplasmosis. J Pediatric Infect Dis Soc 2014; 3 Suppl 1: S30-35.
[7]	Sterkers Y, Pratlong F, et al. Novel interpretation of molecular diagnosis of congenital toxoplasmosis according to gestational age at the time of maternal infection. J Clin Microbiol 2012; 50 (12): 3944-3951.
[8]	Adams Waldorf KM and McAdams RM. Influence of infection during pregnancy on fetal development. Reproduction 2013; 146 (5): R151-162.
[9]	Fowler KB and Boppana SB. Congenital cytomegalovirus (CMV) infection and hearing deficit. J Clin Virol 2006; 35 (2): 226-231.
[10]	Delforge ML, Desomberg L, et al. Evaluation of the new LIAISON ((R)) CMV IgG, IgM and IgG Avidity II assays. J Clin Virol 2015; 72: 42-45.
[11]	Montoya JG. Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J Infect Dis 2002; 185 Suppl 1: S73-82.
[12]	Wu D, Wu Y, et al. Evaluation of a novel array-based toxoplasma, rubella, cytomegalovirus, and herpes simplex virus IgG enzyme linked immunosorbent assay and its comparison with virion/serion enzyme linked immunosorbent assays. Ann Lab Med 2014; 34 (1): 38-42.
[13]	Petersen E, Borobio MV, et al. European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index. J Clin Microbiol 2005; 43 (4): 1570-1574.
[14]	Khan K, Khan W. 2018. Congenital toxoplasmosis: an overview of the neurological and ocular manifestations. Parasitology International, 67, 715–721.

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APA Style

Genco Francesca, Meroni Valeria, De Silvestri Annalisa, Bouthry Elise. (2020). Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening. American Journal of BioScience, 8(1), 15-19. https://doi.org/10.11648/j.ajbio.20200801.13

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ACS Style

Genco Francesca; Meroni Valeria; De Silvestri Annalisa; Bouthry Elise. Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening. Am. J. BioScience 2020, 8(1), 15-19. doi: 10.11648/j.ajbio.20200801.13

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AMA Style

Genco Francesca, Meroni Valeria, De Silvestri Annalisa, Bouthry Elise. Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening. Am J BioScience. 2020;8(1):15-19. doi: 10.11648/j.ajbio.20200801.13

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@article{10.11648/j.ajbio.20200801.13,
  author = {Genco Francesca and Meroni Valeria and De Silvestri Annalisa and Bouthry Elise},
  title = {Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening},
  journal = {American Journal of BioScience},
  volume = {8},
  number = {1},
  pages = {15-19},
  doi = {10.11648/j.ajbio.20200801.13},
  url = {https://doi.org/10.11648/j.ajbio.20200801.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20200801.13},
  abstract = {Toxoplasma gondii (T. gondii) and cytomegalovirus (CMV) infections are typically asymptomatic infections, but they can have serious consequences mainly in newborns and immunocompromised patients. In many parts of the world, these infections are routinely screened during pregnancy (toxoplasmosis) and, in others, high-risk individuals are tested using fully automated screening assays. In this study, we investigated the performance of the three fully automated immunoassays, LIAISON® XL DiaSorin, Abbott Architect and Roche Cobas®, for the determination of specific IgG antibodies to Cytomegalovirus and Toxoplasma gondii in human serum or plasma samples in terms of prevalence of CMV and Toxo IgG detected, and both sensitivity and specificity. Performance of the LIAISON® assays was investigated compared to two other assays, ARCHITECT (CMV IgG and Toxo IgG assays) and Cobas® (CMV IgG and Toxo IgG assays). Discrepant anti CMV IgG and anti Toxoplasma IgG samples were tested for IgM to CMV and Toxoplasma to exclude early acute infection where IgG could be detected differently by the methods. Overall, for both CMV IgG and Toxo IgG, the LIAISON® assay was better than both the Cobas® and ARCHITECT assays in terms of CMV and Toxo IgG detected, and both diagnostic sensitivity and specificity performance although the difference is statistically significant only compared to Cobas®.},
 year = {2020}
}

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TY  - JOUR
T1  - Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening
AU  - Genco Francesca
AU  - Meroni Valeria
AU  - De Silvestri Annalisa
AU  - Bouthry Elise
Y1  - 2020/04/13
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbio.20200801.13
DO  - 10.11648/j.ajbio.20200801.13
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 15
EP  - 19
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20200801.13
AB  - Toxoplasma gondii (T. gondii) and cytomegalovirus (CMV) infections are typically asymptomatic infections, but they can have serious consequences mainly in newborns and immunocompromised patients. In many parts of the world, these infections are routinely screened during pregnancy (toxoplasmosis) and, in others, high-risk individuals are tested using fully automated screening assays. In this study, we investigated the performance of the three fully automated immunoassays, LIAISON® XL DiaSorin, Abbott Architect and Roche Cobas®, for the determination of specific IgG antibodies to Cytomegalovirus and Toxoplasma gondii in human serum or plasma samples in terms of prevalence of CMV and Toxo IgG detected, and both sensitivity and specificity. Performance of the LIAISON® assays was investigated compared to two other assays, ARCHITECT (CMV IgG and Toxo IgG assays) and Cobas® (CMV IgG and Toxo IgG assays). Discrepant anti CMV IgG and anti Toxoplasma IgG samples were tested for IgM to CMV and Toxoplasma to exclude early acute infection where IgG could be detected differently by the methods. Overall, for both CMV IgG and Toxo IgG, the LIAISON® assay was better than both the Cobas® and ARCHITECT assays in terms of CMV and Toxo IgG detected, and both diagnostic sensitivity and specificity performance although the difference is statistically significant only compared to Cobas®.
VL  - 8
IS  - 1
ER  -

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Author Information

Genco Francesca

Microbiology and Virology Unit, Fondazione Istituto di Ricerca e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy
Meroni Valeria

Department of Internal Medicine and Medical Therapy, University of Pavia, Pavia, Italy
De Silvestri Annalisa

Clinical Epidemiology and Biostatistics Unit, Fondazione Istituto di Ricerca e Cura a Carattere Scientifico Policlinico San Matteo, Pavia, Italy
Bouthry Elise

Lab. CERBA Saint-Ouen-l'Aum?ne, Paris, France

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APA Style

Genco Francesca, Meroni Valeria, De Silvestri Annalisa, Bouthry Elise. (2020). Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening. American Journal of BioScience, 8(1), 15-19. https://doi.org/10.11648/j.ajbio.20200801.13

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ACS Style

Genco Francesca; Meroni Valeria; De Silvestri Annalisa; Bouthry Elise. Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening. Am. J. BioScience 2020, 8(1), 15-19. doi: 10.11648/j.ajbio.20200801.13

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AMA Style

Genco Francesca, Meroni Valeria, De Silvestri Annalisa, Bouthry Elise. Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening. Am J BioScience. 2020;8(1):15-19. doi: 10.11648/j.ajbio.20200801.13

Copy | Download

@article{10.11648/j.ajbio.20200801.13,
  author = {Genco Francesca and Meroni Valeria and De Silvestri Annalisa and Bouthry Elise},
  title = {Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening},
  journal = {American Journal of BioScience},
  volume = {8},
  number = {1},
  pages = {15-19},
  doi = {10.11648/j.ajbio.20200801.13},
  url = {https://doi.org/10.11648/j.ajbio.20200801.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20200801.13},
  abstract = {Toxoplasma gondii (T. gondii) and cytomegalovirus (CMV) infections are typically asymptomatic infections, but they can have serious consequences mainly in newborns and immunocompromised patients. In many parts of the world, these infections are routinely screened during pregnancy (toxoplasmosis) and, in others, high-risk individuals are tested using fully automated screening assays. In this study, we investigated the performance of the three fully automated immunoassays, LIAISON® XL DiaSorin, Abbott Architect and Roche Cobas®, for the determination of specific IgG antibodies to Cytomegalovirus and Toxoplasma gondii in human serum or plasma samples in terms of prevalence of CMV and Toxo IgG detected, and both sensitivity and specificity. Performance of the LIAISON® assays was investigated compared to two other assays, ARCHITECT (CMV IgG and Toxo IgG assays) and Cobas® (CMV IgG and Toxo IgG assays). Discrepant anti CMV IgG and anti Toxoplasma IgG samples were tested for IgM to CMV and Toxoplasma to exclude early acute infection where IgG could be detected differently by the methods. Overall, for both CMV IgG and Toxo IgG, the LIAISON® assay was better than both the Cobas® and ARCHITECT assays in terms of CMV and Toxo IgG detected, and both diagnostic sensitivity and specificity performance although the difference is statistically significant only compared to Cobas®.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Comparison of Three Diagnostic Methods for the Detection of Cytomegalovirus and Toxoplasma gondii IgG Antibodies at Prenatal Screening
AU  - Genco Francesca
AU  - Meroni Valeria
AU  - De Silvestri Annalisa
AU  - Bouthry Elise
Y1  - 2020/04/13
PY  - 2020
N1  - https://doi.org/10.11648/j.ajbio.20200801.13
DO  - 10.11648/j.ajbio.20200801.13
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 15
EP  - 19
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20200801.13
AB  - Toxoplasma gondii (T. gondii) and cytomegalovirus (CMV) infections are typically asymptomatic infections, but they can have serious consequences mainly in newborns and immunocompromised patients. In many parts of the world, these infections are routinely screened during pregnancy (toxoplasmosis) and, in others, high-risk individuals are tested using fully automated screening assays. In this study, we investigated the performance of the three fully automated immunoassays, LIAISON® XL DiaSorin, Abbott Architect and Roche Cobas®, for the determination of specific IgG antibodies to Cytomegalovirus and Toxoplasma gondii in human serum or plasma samples in terms of prevalence of CMV and Toxo IgG detected, and both sensitivity and specificity. Performance of the LIAISON® assays was investigated compared to two other assays, ARCHITECT (CMV IgG and Toxo IgG assays) and Cobas® (CMV IgG and Toxo IgG assays). Discrepant anti CMV IgG and anti Toxoplasma IgG samples were tested for IgM to CMV and Toxoplasma to exclude early acute infection where IgG could be detected differently by the methods. Overall, for both CMV IgG and Toxo IgG, the LIAISON® assay was better than both the Cobas® and ARCHITECT assays in terms of CMV and Toxo IgG detected, and both diagnostic sensitivity and specificity performance although the difference is statistically significant only compared to Cobas®.
VL  - 8
IS  - 1
ER  -

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