Hematological Parameters and Serum Biochemistry of <i>Clarias gariepinus</i> Juveniles Exposed to Vestaline<sup>®</sup> (Pendimethalin) Herbicide

Odo Joel Inya; Amuta Elizabeth; Umeh Ebele; Azua Emmanuel; Odo Edna

doi:doi:10.11648/j.ajbio.20190701.14

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Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline^® (Pendimethalin) Herbicide

Odo Joel Inya, Amuta Elizabeth, Umeh Ebele, Azua Emmanuel, Odo Edna

Published in American Journal of BioScience (Volume 7, Issue 1)

Received: 13 June 2018 Accepted: 7 July 2018 Published: 13 April 2019

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Abstract

The study was carried out to determine the effects of sub lethal concentrations of vestaline^® (pendimethalin) herbicide to haematological and serum biochemistry of juveniles of African catfish (Clarias gariepinus) using static bioassays. The toxicity was carried out using 180 healthy and active fish of mean weight of 27.97±0.03 g which were divided into six treatments with each treatment having ten fish and the setup was in triplicate. The fish were exposed to (1.89 mg/l, 3.79 mg/l, 5.68mg/l, 7.57mg/l. 9.46mg/l and 0.00 mg/l)) concentrations of Vestaline^® (Pendimethalin) herbicide for a period of eight weeks during which the fish were fed at 5% of their body weight twice daily. Data obtained were analyzed for descriptive statistics (mean and standard error of means).The data were further subjected to analysis of variance (ANOVA) at P< 0.05 using Minitab. Results showed that Haematological parameters reduced significantly (P<0.05) in amount of HCT, Hb and RBC with increase in concentration an indication of Anaemia. While WBC increased significantly (P<0.05) with increase in concentration. All were dose dependant. The results of Serum Biochemistry showed significant difference (P<0.05) in all the parameters determined. There was significant (P<0.05) decrease in Total protein and Albumin while Cholesterol, Alanine aminoTransferase, Aspartate aminoTransferase and Creatine all increased significantly (P<0.05) with increase in concentration. Vestaline^® (Pendimethalin) herbicide from this study is toxic on Clarias gariepinus juveniles so its use around aquatic environment should be done with care.

Published in	American Journal of BioScience (Volume 7, Issue 1)
DOI	10.11648/j.ajbio.20190701.14
Page(s)	25-30
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Clarias gariepinus, Vestalin^® (Pendimethalin), Hematology

References

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APA Style

Odo Joel Inya, Amuta Elizabeth, Umeh Ebele, Azua Emmanuel, Odo Edna. (2019). Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide. American Journal of BioScience, 7(1), 25-30. https://doi.org/10.11648/j.ajbio.20190701.14

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ACS Style

Odo Joel Inya; Amuta Elizabeth; Umeh Ebele; Azua Emmanuel; Odo Edna. Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide. Am. J. BioScience 2019, 7(1), 25-30. doi: 10.11648/j.ajbio.20190701.14

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AMA Style

Odo Joel Inya, Amuta Elizabeth, Umeh Ebele, Azua Emmanuel, Odo Edna. Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide. Am J BioScience. 2019;7(1):25-30. doi: 10.11648/j.ajbio.20190701.14

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@article{10.11648/j.ajbio.20190701.14,
  author = {Odo Joel Inya and Amuta Elizabeth and Umeh Ebele and Azua Emmanuel and Odo Edna},
  title = {Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide},
  journal = {American Journal of BioScience},
  volume = {7},
  number = {1},
  pages = {25-30},
  doi = {10.11648/j.ajbio.20190701.14},
  url = {https://doi.org/10.11648/j.ajbio.20190701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20190701.14},
  abstract = {The study was carried out to determine the effects of sub lethal concentrations of vestaline® (pendimethalin) herbicide to haematological and serum biochemistry of juveniles of African catfish (Clarias gariepinus) using static bioassays. The toxicity was carried out using 180 healthy and active fish of mean weight of 27.97±0.03 g which were divided into six treatments with each treatment having ten fish and the setup was in triplicate. The fish were exposed to (1.89 mg/l, 3.79 mg/l, 5.68mg/l, 7.57mg/l. 9.46mg/l and 0.00 mg/l)) concentrations of Vestaline® (Pendimethalin) herbicide for a period of eight weeks during which the fish were fed at 5% of their body weight twice daily. Data obtained were analyzed for descriptive statistics (mean and standard error of means).The data were further subjected to analysis of variance (ANOVA) at P0.05 using Minitab. Results showed that Haematological parameters reduced significantly (P® (Pendimethalin) herbicide from this study is toxic on Clarias gariepinus juveniles so its use around aquatic environment should be done with care.},
 year = {2019}
}

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TY  - JOUR
T1  - Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide
AU  - Odo Joel Inya
AU  - Amuta Elizabeth
AU  - Umeh Ebele
AU  - Azua Emmanuel
AU  - Odo Edna
Y1  - 2019/04/13
PY  - 2019
N1  - https://doi.org/10.11648/j.ajbio.20190701.14
DO  - 10.11648/j.ajbio.20190701.14
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 25
EP  - 30
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20190701.14
AB  - The study was carried out to determine the effects of sub lethal concentrations of vestaline® (pendimethalin) herbicide to haematological and serum biochemistry of juveniles of African catfish (Clarias gariepinus) using static bioassays. The toxicity was carried out using 180 healthy and active fish of mean weight of 27.97±0.03 g which were divided into six treatments with each treatment having ten fish and the setup was in triplicate. The fish were exposed to (1.89 mg/l, 3.79 mg/l, 5.68mg/l, 7.57mg/l. 9.46mg/l and 0.00 mg/l)) concentrations of Vestaline® (Pendimethalin) herbicide for a period of eight weeks during which the fish were fed at 5% of their body weight twice daily. Data obtained were analyzed for descriptive statistics (mean and standard error of means).The data were further subjected to analysis of variance (ANOVA) at P0.05 using Minitab. Results showed that Haematological parameters reduced significantly (P® (Pendimethalin) herbicide from this study is toxic on Clarias gariepinus juveniles so its use around aquatic environment should be done with care.
VL  - 7
IS  - 1
ER  -

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Author Information

Odo Joel Inya

Department of Fisheries and Aquaculture, University of Agriculture, Makurdi, Nigeria
Amuta Elizabeth

Department of Biological Sciences, University of Agriculture, Makurdi, Nigeria
Umeh Ebele

Department of Biological Sciences, University of Agriculture, Makurdi, Nigeria
Azua Emmanuel

Department of Biological Sciences, University of Agriculture, Makurdi, Nigeria
Odo Edna

Department of Fisheries and Aquaculture, University of Agriculture, Makurdi, Nigeria

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Odo Joel Inya, Amuta Elizabeth, Umeh Ebele, Azua Emmanuel, Odo Edna. (2019). Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide. American Journal of BioScience, 7(1), 25-30. https://doi.org/10.11648/j.ajbio.20190701.14

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ACS Style

Odo Joel Inya; Amuta Elizabeth; Umeh Ebele; Azua Emmanuel; Odo Edna. Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide. Am. J. BioScience 2019, 7(1), 25-30. doi: 10.11648/j.ajbio.20190701.14

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AMA Style

Odo Joel Inya, Amuta Elizabeth, Umeh Ebele, Azua Emmanuel, Odo Edna. Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide. Am J BioScience. 2019;7(1):25-30. doi: 10.11648/j.ajbio.20190701.14

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@article{10.11648/j.ajbio.20190701.14,
  author = {Odo Joel Inya and Amuta Elizabeth and Umeh Ebele and Azua Emmanuel and Odo Edna},
  title = {Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide},
  journal = {American Journal of BioScience},
  volume = {7},
  number = {1},
  pages = {25-30},
  doi = {10.11648/j.ajbio.20190701.14},
  url = {https://doi.org/10.11648/j.ajbio.20190701.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbio.20190701.14},
  abstract = {The study was carried out to determine the effects of sub lethal concentrations of vestaline® (pendimethalin) herbicide to haematological and serum biochemistry of juveniles of African catfish (Clarias gariepinus) using static bioassays. The toxicity was carried out using 180 healthy and active fish of mean weight of 27.97±0.03 g which were divided into six treatments with each treatment having ten fish and the setup was in triplicate. The fish were exposed to (1.89 mg/l, 3.79 mg/l, 5.68mg/l, 7.57mg/l. 9.46mg/l and 0.00 mg/l)) concentrations of Vestaline® (Pendimethalin) herbicide for a period of eight weeks during which the fish were fed at 5% of their body weight twice daily. Data obtained were analyzed for descriptive statistics (mean and standard error of means).The data were further subjected to analysis of variance (ANOVA) at P0.05 using Minitab. Results showed that Haematological parameters reduced significantly (P® (Pendimethalin) herbicide from this study is toxic on Clarias gariepinus juveniles so its use around aquatic environment should be done with care.},
 year = {2019}
}

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TY  - JOUR
T1  - Hematological Parameters and Serum Biochemistry of Clarias gariepinus Juveniles Exposed to Vestaline® (Pendimethalin) Herbicide
AU  - Odo Joel Inya
AU  - Amuta Elizabeth
AU  - Umeh Ebele
AU  - Azua Emmanuel
AU  - Odo Edna
Y1  - 2019/04/13
PY  - 2019
N1  - https://doi.org/10.11648/j.ajbio.20190701.14
DO  - 10.11648/j.ajbio.20190701.14
T2  - American Journal of BioScience
JF  - American Journal of BioScience
JO  - American Journal of BioScience
SP  - 25
EP  - 30
PB  - Science Publishing Group
SN  - 2330-0167
UR  - https://doi.org/10.11648/j.ajbio.20190701.14
AB  - The study was carried out to determine the effects of sub lethal concentrations of vestaline® (pendimethalin) herbicide to haematological and serum biochemistry of juveniles of African catfish (Clarias gariepinus) using static bioassays. The toxicity was carried out using 180 healthy and active fish of mean weight of 27.97±0.03 g which were divided into six treatments with each treatment having ten fish and the setup was in triplicate. The fish were exposed to (1.89 mg/l, 3.79 mg/l, 5.68mg/l, 7.57mg/l. 9.46mg/l and 0.00 mg/l)) concentrations of Vestaline® (Pendimethalin) herbicide for a period of eight weeks during which the fish were fed at 5% of their body weight twice daily. Data obtained were analyzed for descriptive statistics (mean and standard error of means).The data were further subjected to analysis of variance (ANOVA) at P0.05 using Minitab. Results showed that Haematological parameters reduced significantly (P® (Pendimethalin) herbicide from this study is toxic on Clarias gariepinus juveniles so its use around aquatic environment should be done with care.
VL  - 7
IS  - 1
ER  -

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